Perepelov A V, Shashkov A S, Babichka D, Senchenkova S N, Bartodziejska B, Rozalski A, Knirel Y A
Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, 117913, Russia.
Biochemistry (Mosc). 2000 Sep;65(9):1055-9.
An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus vulgaris O23 (strain PrK 44/57) and found to contain 2-acetamido-2-deoxy-D-galactose, 2-acetamido-2-deoxy-D-glucose, and D-galacturonic acid. Based on 1H- and 13C-NMR spectroscopic studies, including two-dimensional correlation spectroscopy (COSY), total correlation spectroscopy (TOCSY), nuclear Overhauser effect spectroscopy (NOESY), and 1H,13C heteronuclear multiple-quantum coherence (HMQC) experiments, the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was established: [figure], where the degree of O-acetylation of the terminal GalA residue at position 4 is about 80%. A structural similarity of the O-specific polysaccharides of P. vulgaris O23 and P. mirabilis O23 is discussed.
通过对普通变形杆菌O23（菌株PrK 44/57）的脂多糖进行温和酸降解，获得了一种酸性O-特异性多糖，发现其含有2-乙酰氨基-2-脱氧-D-半乳糖、2-乙酰氨基-2-脱氧-D-葡萄糖和D-半乳糖醛酸。基于1H-和13C-NMR光谱研究，包括二维相关光谱（COSY）、全相关光谱（TOCSY）、核Overhauser效应光谱（NOESY）和1H,13C异核多量子相干（HMQC）实验，确定了该多糖支链四糖重复单元的以下结构：[图]，其中位置4处末端GalA残基的O-乙酰化程度约为80%。讨论了普通变形杆菌O23和奇异变形杆菌O23的O-特异性多糖的结构相似性。
