Franzini C, Panseri C
Clin Chim Acta. 1976 May 17;69(1):73-9. doi: 10.1016/0009-8981(76)90473-3.
From a concentrated solution of bilirubin in dimethylsulfoxide (about 13 mmol/1, standardized spectrophotometrically) and a serum diluted 1/3 (whose native bilirubin is lowered by exposure to phototherapy lamps) a bilirubin-enriched serum is prepared (bilirubin concentration about 130 mumol/1 = 7.5 mg/dl) wherein all the bilrubin is bound to albumin. Such an enriched serum behaves in the diazo-reaction in the same way as a bilirubin-enriched serum prepared with undiluted serum and can, therefore, be used as a working standard in serum bilirubin assays.
从胆红素在二甲基亚砜中的浓缩溶液（约13 mmol/1，通过分光光度法标准化）和稀释1/3的血清（其天然胆红素因暴露于光疗灯下而降低）制备富含胆红素的血清（胆红素浓度约130 μmol/1 = 7.5 mg/dl），其中所有胆红素均与白蛋白结合。这种富含胆红素的血清在重氮反应中的表现与用未稀释血清制备的富含胆红素的血清相同，因此可作为血清胆红素测定的工作标准品。
