犬5-羟色胺受体1A基因（htr1A）的分离与鉴定。

Isolation and characterization of the canine serotonin receptor 1A gene (htr1A).

作者信息

van den Berg L, Versteeg S A, van Oost B A

机构信息

Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 8, 3584 CM Utrecht, The Netherlands.

出版信息

J Hered. 2003 Jan-Feb;94(1):49-56. doi: 10.1093/jhered/esg013.

DOI:10.1093/jhered/esg013

PMID:12692162

Abstract

Although the serotonergic system and htr1A have been studied extensively, little is known about the canine serotonin receptor 1A. We are interested in this receptor in the dog because it is likely to be involved in behavioral disorders such as anxiety. Therefore, we isolated a canine bacterial artificial chromosome (BAC) clone containing htr1A, and, with the help of this clone, the complete canine coding sequence of this gene was determined. Radiation hybrid (RH) mapping showed that htr1A is part of a conserved linkage group also including the survival of motor neuron 1 (smn1) gene. Htr1A is estimated to be located about 7.3 Mb from smn1 on cfa02. In addition, we report a possible breed-specific variant of the gene in four golden retrievers.

摘要

尽管血清素能系统和5-羟色胺受体1A（htr1A）已得到广泛研究，但对犬类血清素受体1A却知之甚少。我们之所以对犬类的这种受体感兴趣，是因为它可能与诸如焦虑等行为障碍有关。因此，我们分离出了一个包含htr1A的犬类细菌人工染色体（BAC）克隆，并借助该克隆确定了该基因完整的犬类编码序列。辐射杂种（RH）图谱显示，htr1A是一个保守连锁群的一部分，该连锁群还包括运动神经元存活蛋白1（smn1）基因。据估计，htr1A在犬类第2号染色体（cfa02）上距离smn1约7.3兆碱基对（Mb）。此外，我们报告了在四只金毛猎犬中该基因可能存在的品种特异性变异。