犬转谷氨酰胺酶1基因的DNA序列及物理图谱

DNA sequence and physical mapping of the canine transglutaminase 1 gene.

作者信息

Credille K M, Venta P J, Breen M, Lowe J K, Murphy K E, Ostrander E A, Galibert F, Dunstan R W

机构信息

Department of Veterinary Pathobiology, Texas A&M University, College Station, TX 77843-4467, USA.

出版信息

Cytogenet Cell Genet. 2001;93(1-2):73-6. doi: 10.1159/000056952.

DOI:10.1159/000056952

PMID:11474183

Abstract

The transglutaminase 1 gene (TGM1) encodes an enzyme necessary for cross-linking the structural proteins that form the cornified envelope, an essential component of the outermost layer of the skin, the stratum corneum. Reported here is the complete coding region of canine TGM1, its chromosome localization, and its map position in the integrated canine linkage-radiation hybrid map. Canine TGM1 consists of 2,448 nucleotides distributed over 15 exons. The nucleotide sequence has 90% identity to human TGM1. The deduced canine TGM1 protein is 816 amino acids long and is 92% identical to human TGM1. Using fluorescence in situ hybridization, we localized canine TGM1 to dog (Canis familiaris) chromosome 8 (CFA 8q). Canine TGM1 localized to CFA 8 on the integrated linkage-radiation hybrid map in the interval FH2149-MYH7. Characterizing the coding region of canine TGM1 is a first step in examining the role of this enzyme in normal and defective cornification in the dog.

摘要

转谷氨酰胺酶1基因（TGM1）编码一种交联结构蛋白所必需的酶，这些结构蛋白构成了角质包膜，而角质包膜是皮肤最外层即角质层的重要组成部分。本文报道了犬TGM1的完整编码区、其染色体定位以及在整合的犬类连锁-辐射杂种图谱中的图谱位置。犬TGM1由分布在15个外显子上的2448个核苷酸组成。该核苷酸序列与人类TGM1有90%的同一性。推导的犬TGM1蛋白长816个氨基酸，与人类TGM1有92%的同一性。利用荧光原位杂交技术，我们将犬TGM1定位到犬（家犬）的8号染色体（CFA 8q）上。在整合的连锁-辐射杂种图谱上，犬TGM1定位在CFA 8的FH2149-MYH7区间。确定犬TGM1的编码区是研究该酶在犬正常和缺陷性角质化过程中作用的第一步。