Afset Jan Egil, Maeland Johan A
Department of Microbiology, School of Medicine, Norwegian University of Science and Technology, Trondheim, Norway.
Scand J Infect Dis. 2003;35(2):84-9. doi: 10.1080/0036554021000026980.
Staphylococcus aureus (SA) isolates (n = 255) from outpatients with skin and soft-tissue infections were collected in 3 different areas in Norway. Group A streptococci (GAS, n = 68) were isolated from skin or pharyngotonsillar specimens from outpatients. Minimum inhibitory concentrations (MIC) of bacitracin, fusidic acid and mupirocin were tested using the E-test. Pulsed field gel electrophoresis (PFGE) patterns of fusidic acid-sensitive (FusS) and -resistant (FusR) SA were compared. All GAS isolates showed MIC of bacitracin of < or = 1.0 mg/l, of mupirocin of < or = 0.125 mg/l and of fusidic acid 1.0-4.0 mg/l. All the SA showed MIC of mupirocin < or = 0.5 mg/l and of bacitracin of > or = 2.0 mg/l, 91% with MIC > or = 16 mg/l. FusR was shown by 32.5% of the SA strains with similar prevalence rates in 3 different geographical areas of Norway. One particular PFGE pattern (type 1) was shown by 76% of the FusR SA. SA of type 1 belonged to phage group II and produced exfoliative toxins. Thus, the results demonstrated a high prevalence of FusR among SA causing skin infections and that this was mainly due to dissemination of clonally related FusR SA.
从挪威3个不同地区的门诊皮肤和软组织感染患者中收集了255株金黄色葡萄球菌（SA）分离株。从门诊患者的皮肤或咽扁桃体标本中分离出68株A组链球菌（GAS）。使用E-test检测杆菌肽、夫西地酸和莫匹罗星的最低抑菌浓度（MIC）。比较了对夫西地酸敏感（FusS）和耐药（FusR）的SA的脉冲场凝胶电泳（PFGE）图谱。所有GAS分离株显示杆菌肽的MIC≤1.0mg/l，莫匹罗星的MIC≤0.125mg/l，夫西地酸的MIC为1.0 - 4.0mg/l。所有SA显示莫匹罗星的MIC≤0.5mg/l，杆菌肽的MIC≥2.0mg/l，91%的菌株MIC≥16mg/l。32.5%的SA菌株表现出夫西地酸耐药（FusR），在挪威3个不同地理区域的流行率相似。76%的FusR SA呈现一种特定的PFGE图谱（1型）。1型SA属于噬菌体II组，可产生剥脱毒素。因此，结果表明在引起皮肤感染的SA中夫西地酸耐药（FusR）的发生率很高，这主要是由于克隆相关的FusR SA的传播所致。
