Centre for Biomedical Sciences, Department of Applied Sciences, Cardiff School of Health Sciences, University of Wales Institute Cardiff, Western Avenue, Cardiff CF5 2YB, UK.
Eur J Clin Microbiol Infect Dis. 2010 Oct;29(10):1237-41. doi: 10.1007/s10096-010-0992-1. Epub 2010 Jun 13.
Clinical use of honey in the topical treatment of wounds has increased in Europe and North America since licensed wound care products became available in 2004 and 2007, respectively. Honey-resistant bacteria have not been isolated from wounds, but there is a need to investigate whether honey has the potential to select for honey resistance. Two cultures of bacteria from reference collections (Staphylococcus aureus NCTC 10017 and Pseudomonas aeruginosa ATCC 27853) and four cultures isolated from wounds (Escherichia coli, methicillin-resistant S. aureus (MRSA), Pseudomonas aeruginosa and S. epidermidis) were exposed to sub-lethal concentrations of manuka honey in continuous and stepwise training experiments to determine whether the susceptibility to honey diminished. Reduced susceptibilities to manuka honey in the test organisms during long-term stepwise resistance training were found, but these changes were not permanent and honey-resistant mutants were not detected. The risk of bacteria acquiring resistance to honey will be low if high concentrations are maintained clinically.
自 2004 年和 2007 年分别有许可的伤口护理产品上市以来，蜂蜜在欧洲和北美局部伤口治疗中的临床应用有所增加。尚未从伤口中分离出抗蜂蜜细菌，但有必要调查蜂蜜是否有可能选择产生抗蜂蜜性。从参考培养物（金黄色葡萄球菌 NCTC 10017 和铜绿假单胞菌 ATCC 27853）中分离出的两种细菌培养物和从伤口中分离出的四种培养物（大肠杆菌、耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌和表皮葡萄球菌）被暴露于亚致死浓度的麦卢卡蜂蜜在连续和逐步训练实验中，以确定对蜂蜜的敏感性是否减弱。在长期逐步耐药训练过程中，测试生物对麦卢卡蜂蜜的敏感性降低，但这些变化不是永久性的，也没有检测到抗蜂蜜突变体。如果临床上保持高浓度，细菌获得对蜂蜜的耐药性的风险将很低。
