Thaweboon Sroisiri, Chunhabundit Panjit, Surarit Rudee, Swasdison Somporn, Suppukpatana Prapan
Department of Microbiology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2002 Sep;33(3):654-61.
Teeth have been recognized as providing a useful long-term record of lead (Pb2+) uptake. However, information regarding the effects of lead on dental pulp tissue cells that foster dentinogenesis is scarce. This study investigated the effects of lead on dental pulp tissue using human dental pulp fibroblasts in vitro. Dental pulp cells from the teeth of young patients (aged 17-24 years) were cultured and subsequently treated with lead glutamate. It was shown that, in serum-free conditions, all the tested concentrations of lead (4.5 x 10(-5) M, 4.5 x 10(-6) M, and 4.5 x 10(-7) M) significantly increased pulpal cell proliferation. In the presence of 2% fetal bovine serum, increasing cell proliferation was observed only after exposure to a lead concentration of 4.5 x 10(-5) M. However, protein, procollagen type I, and osteocalcin productions were significantly decreased. The alteration of cell population and protein production of affected human dental pulp shown in this study are toxic effects of the lead.
牙齿已被认为能提供关于铅（Pb2+）摄取的有用长期记录。然而，关于铅对促进牙本质形成的牙髓组织细胞影响的信息却很匮乏。本研究在体外使用人牙髓成纤维细胞研究了铅对牙髓组织的影响。培养了年轻患者（17 - 24岁）牙齿的牙髓细胞，随后用谷氨酸铅进行处理。结果表明，在无血清条件下，所有测试浓度的铅（4.5×10(-5) M、4.5×10(-6) M和4.5×10(-7) M）均显著增加了牙髓细胞增殖。在存在2%胎牛血清的情况下，仅在暴露于4.5×10(-5) M的铅浓度后才观察到细胞增殖增加。然而，蛋白质、I型前胶原蛋白和骨钙素的产生均显著减少。本研究中受影响的人牙髓细胞群体和蛋白质产生的改变是铅的毒性作用。
