Barkhordar Rahmat A, Ghani Q Perveen, Russell Thomas R, Hussain M Zamirul
Department of Preventive & Restorative Dental Sciences, School of Dentistry, University of California, San Francisco 94143, USA.
J Endod. 2002 Mar;28(3):157-9. doi: 10.1097/00004770-200203000-00003.
Immunopathologic reactions play a significant role in inflammatory diseases of dental pulp. Interleukin-1beta (IL-1beta) is recognized as a key player in mediating cellular immune response. In this study, we measured the content of IL-1beta and its effect on collagen synthesis in cultures of fibroblasts derived from healthy and diseased dental pulps. We found that diseased pulp fibroblasts contain 2.5-fold greater amounts of IL-1beta and synthesized 80% greater amounts of collagen compared with healthy pulp fibroblasts. However, exogenous IL-1beta failed to stimulate collagen synthesis by diseased fibroblasts, whereas collagen synthesis by healthy pulp fibroblasts was stimulated by more than 2-fold. These observations imply that pulp disease induces abnormalities associated with fibroblast response toward IL-1beta.
免疫病理反应在牙髓炎症性疾病中起重要作用。白细胞介素-1β（IL-1β）被认为是介导细胞免疫反应的关键因子。在本研究中，我们检测了健康和患病牙髓来源的成纤维细胞培养物中IL-1β的含量及其对胶原蛋白合成的影响。我们发现，与健康牙髓成纤维细胞相比，患病牙髓成纤维细胞所含的IL-1β量高2.5倍，胶原蛋白合成量高80%。然而，外源性IL-1β未能刺激患病成纤维细胞的胶原蛋白合成，而健康牙髓成纤维细胞的胶原蛋白合成则被刺激了2倍多。这些观察结果表明，牙髓疾病会诱导与成纤维细胞对IL-1β反应相关的异常。
