Furukawa S, Shimoda M, Hayakawa I
Laboratory of Food Process Engineering, Faculty of Agriculture, Kyushu University, Fukuoka-shi, Japan.
J Appl Microbiol. 2003;94(5):836-41. doi: 10.1046/j.1365-2672.2003.01913.x.
The mechanism of the inactivation of Bacillus subtilis spores by reciprocal pressurization (RP) was unclear. Therefore, the mechanism was investigated.
To investigate the effects of RP and continuous pressurization (CP) treatments on the inactivation and injury of B. subtilis spores, spores were treated at 25, 35, 45 and 55 degrees C under 200, 300 and 400 MPa. RP treatment was effective in injuring and inactivating spores. Scanning electron microscopy and transmission electron microscopy observation showed that spores treated by RP treatment were more morphologically and structurally changed than the ones treated by CP treatment. There were significant differences between the release of dipicolinic acid (pyridine-2,6-dicarboxylic acid) by RP and CP treatments. From this result, it was concluded that the core fraction was released into the spore suspension.
The mechanism of RP treatment is believed to work as follows: hydrostatic pressure treatment initiated germination of bacterial spores, and the repeated rapid decompression caused disruption, injury and inactivation of the germinated spores.
This study indicated that the physical injury of bacterial spores was effective to inactivate the bacterial spores through the disruption of spores and leakage of their contents.
相互加压（RP）灭活枯草芽孢杆菌孢子的机制尚不清楚。因此，对该机制进行了研究。
为研究RP和连续加压（CP）处理对枯草芽孢杆菌孢子灭活和损伤的影响，将孢子在200、300和400MPa下于25、35、45和55℃进行处理。RP处理对孢子的损伤和灭活有效。扫描电子显微镜和透射电子显微镜观察表明，RP处理的孢子在形态和结构上的变化比CP处理的孢子更大。RP和CP处理在吡啶二羧酸（2,6-吡啶二甲酸）释放方面存在显著差异。由此结果得出，核心部分释放到孢子悬浮液中。
RP处理的机制被认为如下：静水压力处理引发细菌孢子萌发，反复快速减压导致萌发孢子破裂、损伤和灭活。
本研究表明，细菌孢子的物理损伤通过破坏孢子及其内容物泄漏有效地灭活了细菌孢子。
