Lemieux George A, De Graffenried Christopher L, Bertozzi Carolyn R
Center for New Directions in Organic Synthesis, Department of Chemistry, Howard Hughes Medical Institute, University of California, Berkeley, California 94720, USA.
J Am Chem Soc. 2003 Apr 23;125(16):4708-9. doi: 10.1021/ja029013y.
Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.
使用生化和生物物理探针进行生物分子的特异性标记是蛋白质组学研究的核心要素。在此，我们描述了一种香豆素 - 膦染料，其在与叠氮化物进行施陶丁格连接反应时会激活香豆素荧光。由于叠氮化物可以代谢性地掺入细胞蛋白质和寡糖中，这种染料可能是分析蛋白质及其翻译后修饰的有用工具。
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