-Pawlak Cornelius R, Ho Ying Jui, Schwarting Rainer K W, Bauhofer Artur
Experimental and Physiological Psychology, Philipps-University of Marburg, Gutenbergstrasse 18, Germany.
Neurosci Lett. 2003 May 8;341(3):205-8. doi: 10.1016/s0304-3940(03)00184-8.
Our previous experiments have shown that adult male Wistar rats can differ systematically in elevated plus-maze (EPM) behaviour, which was related to the neurotransmitter serotonin in the ventral striatum. The EPM serves as a model of anxiety-like behaviour, and there is evidence that interleukin (IL)-2 in the brain may be related to anxiety-like behaviour, and that IL-2 interacts with the striatal serotonergic system. We asked whether EPM behaviour may also be related to constitutive levels of cytokines in the striatum. Based on open arm time in the EPM, male Wistar rats were divided into sub-groups with either low or high anxiety-like behaviour. Then, IL-1beta, IL-2, IL-6, and tumour necrosis factor (TNF)-alpha cDNA levels were measured post mortem in striatal tissues using semi-quantitative, competitive, reverse transcription polymerase chain reaction. Rats with high anxiety-like behaviour in the EPM showed significantly higher levels of IL-2 mRNA compared to those with low anxiety-like behaviour, but did not differ significantly in expression of IL-1beta, IL-6, and TNF-alpha mRNA. These results provide new evidence indicating that specific cytokine patterns in the striatum may be associated with EPM behaviour in adult male Wistar rats.
我们之前的实验表明，成年雄性Wistar大鼠在高架十字迷宫（EPM）行为上可能存在系统性差异，这与腹侧纹状体中的神经递质血清素有关。EPM可作为类似焦虑行为的模型，并且有证据表明大脑中的白细胞介素（IL）-2可能与类似焦虑行为有关，而且IL-2与纹状体血清素能系统相互作用。我们探究了EPM行为是否也与纹状体中细胞因子的组成水平有关。根据EPM中的开臂时间，将雄性Wistar大鼠分为具有低或高类似焦虑行为的亚组。然后，使用半定量、竞争性逆转录聚合酶链反应在死后的纹状体组织中测量IL-1β、IL-2、IL-6和肿瘤坏死因子（TNF）-α的cDNA水平。与具有低类似焦虑行为的大鼠相比，在EPM中具有高类似焦虑行为的大鼠显示出显著更高的IL-2 mRNA水平，但在IL-1β、IL-6和TNF-α mRNA的表达上没有显著差异。这些结果提供了新的证据，表明纹状体中的特定细胞因子模式可能与成年雄性Wistar大鼠的EPM行为有关。
