Maughan S C, Cobbett C S
Department of Genetics, University of Melbourne, 3010, Melbourne, Vic., Australia.
J Biotechnol. 2003 Apr 24;102(2):125-8. doi: 10.1016/s0168-1656(03)00028-2.
Methionine sulfoximine, like phosphinothricin (PPT), the active agent in the herbicide BASTA, is a glutamate analogue that inhibits growth of wildtype Arabidopsis plants through its action on glutamine synthetase. The bar gene, which confers resistance to PPT, also confers resistance to methionine sulfoximine. In this study we show that methionine sulfoximine is an effective and economical alternative to PPT as a selective agent in agar medium.
甲硫氨酸亚砜亚胺，与除草剂“Basta”中的活性成分草丁膦（PPT）一样，是一种谷氨酸类似物，它通过作用于谷氨酰胺合成酶来抑制野生型拟南芥植物的生长。赋予对PPT抗性的bar基因也赋予对甲硫氨酸亚砜亚胺的抗性。在本研究中，我们表明甲硫氨酸亚砜亚胺作为琼脂培养基中的选择剂，是一种有效且经济的替代PPT的物质。
