Oct-1对人FcepsilonRIβ链基因表达的调控

Regulation of human FcepsilonRI beta chain gene expression by Oct-1.

作者信息

Akizawa Yushiro, Nishiyama Chiharu, Hasegawa Masanari, Maeda Keiko, Nakahata Tatsutoshi, Okumura Ko, Ra Chisei, Ogawa Hideoki

机构信息

Atopy (Allergy) Research Center, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.

出版信息

Int Immunol. 2003 May;15(5):549-56. doi: 10.1093/intimm/dxg055.

DOI:10.1093/intimm/dxg055

PMID:12697656

Abstract

The beta chain, a component of the high-affinity receptor for IgE (FcepsilonRI), plays an important role in IgE-mediated allergic reaction. The beta chain accelerates the function of FcepsilonRI by amplification of its surface expression and of signal transduction in effector cells such as mast cells and basophils. Two regulatory regions, +60/+66 and +70/+76, for the human beta chain gene that are required for the cell-type-specific transcriptional activation were identified by transient reporter assay. Electrophoretic mobility shift assay demonstrated that Oct-1 binds both the regions, among which the +70/+76 Oct-1 motif was critical for the transactivation of the beta promoter responsive to Oct-1 overexpression. Regulation of beta chain gene expression is discussed.

摘要

β链是IgE高亲和力受体（FcepsilonRI）的一个组成部分，在IgE介导的过敏反应中起重要作用。β链通过放大其在效应细胞（如肥大细胞和嗜碱性粒细胞）表面的表达以及信号转导来加速FcepsilonRI的功能。通过瞬时报告基因检测，确定了人β链基因的两个调控区域+60/+66和+70/+76，它们是细胞类型特异性转录激活所必需的。电泳迁移率变动分析表明，Oct-1与这两个区域都结合，其中+70/+76 Oct-1基序对于响应Oct-1过表达的β启动子的反式激活至关重要。文中讨论了β链基因表达的调控。