Teramoto Tetsuyuki, Qiu Jianhua, Plumier Jean-Christophe, Moskowitz Michael A
Neuroscience Center, Radiology Department, Massachusetts General Hospital and Neuroscience Program, Harvard Medical School, Boston, MA 02129, USA.
J Clin Invest. 2003 Apr;111(8):1125-32. doi: 10.1172/JCI17170.
EGF promotes proliferation and migration of stem/progenitor cells in the normal adult brain. The effect of epidermal growth factor on neurogenesis in ischemic brain is unknown, however. Here we show that intraventricular administration of EGF and albumin augments 100-fold neuronal replacement in the injured adult mouse striatum after cerebral ischemia. Newly born immature neurons migrate into the ischemic lesion and differentiate into mature parvalbumin-expressing neurons, replacing more than 20% of the interneurons lost by 13 weeks after ischemia and representing 2% of the total BrdU-labeled cells. These data suggest that administration of EGF and albumin could be used to manipulate endogenous neurogenesis in the injured brain and to promote brain self-repair.
表皮生长因子(EGF)可促进正常成年大脑中干细胞/祖细胞的增殖和迁移。然而,表皮生长因子对缺血性脑内神经发生的影响尚不清楚。在此我们表明,脑室内给予表皮生长因子和白蛋白可使成年小鼠脑缺血后损伤的纹状体中的神经元替代增加100倍。新生的未成熟神经元迁移至缺血性损伤部位,并分化为表达小白蛋白的成熟神经元,替代了缺血13周后损失的中间神经元的20%以上,占溴脱氧尿苷(BrdU)标记细胞总数的2%。这些数据表明,给予表皮生长因子和白蛋白可用于调控损伤脑内的内源性神经发生,并促进脑自我修复。