鸭嘴兽SOX2和SOX14的克隆与定位：对SOX B族进化的见解

Cloning and mapping of platypus SOX2 and SOX14: insights into SOX group B evolution.

作者信息

Kirby P J, Waters P D, Delbridge M, Svartman M, Stewart A N, Nagai K, Graves J A M

机构信息

Department of Genetics, La Trobe University, Bundoora, Victoria, Australia.

出版信息

Cytogenet Genome Res. 2002;98(1):96-100. doi: 10.1159/000068539.

DOI:10.1159/000068539

PMID:12584449

Abstract

Group B SOX genes, the closest relatives to the sex-determining gene SRY, are thought to have evolved from a single ancestral SOX B by a series of duplications and translocations. The two SOX B genes SOX2 and SOX14 co-localize to chromosome 3q in humans. SOX2 and SOX14 homologues were cloned and characterized in the platypus, a monotreme mammal distantly related to man. The two genes were found to co-localize to chromosome 1q in this species. Proximity of the two related genes has therefore been conserved for 170 Myr, since humans and platypus diverged. The sequence similarity and conserved synteny of these group B genes provide clues to their origin. A simple model of SOX group B gene evolution is proposed.

摘要

B组SOX基因是与性别决定基因SRY关系最为密切的基因，被认为是通过一系列的复制和易位从单一的祖先SOX B基因进化而来。人类的两个SOX B基因SOX2和SOX14共定位于3号染色体长臂。在与人类亲缘关系较远的单孔目哺乳动物鸭嘴兽中克隆并鉴定了SOX2和SOX14的同源基因。结果发现这两个基因在该物种中共定位于1号染色体长臂。因此，自人类和鸭嘴兽分化以来，这两个相关基因的邻近关系已经保守了1.7亿年。这些B组基因的序列相似性和保守的同线性为它们的起源提供了线索。本文提出了一个简单的SOX B组基因进化模型。