糖耐量受损时胆固醇酯转运蛋白活性增加：与高密度脂蛋白代谢的关系

Increased cholesteryl ester transfer protein activity in impaired glucose tolerance: relationship to high density lipoprotein metabolism.

作者信息

Pietzsch Jens, Fuecker Katja

机构信息

Pathological Biochemistry Group, Department Positron Emission Tomography, Institute of Bioinorganic and Radiopharmaceutical Chemistry, Rossendorf Research Center, P.O. Box 51 01 19, D-01314 Dresden, Germany.

出版信息

Croat Med J. 2003 Apr;44(2):171-7.

DOI:

PMID:12698508

Abstract

AIM

To investigate the mechanisms and dynamics of cholesteryl ester (neutral lipid) transfer protein activity in subjects with impaired glucose tolerance.

METHODS

Eighty six Caucasian subjects were recruited by advertisement from the local population between January 1998 and December 1999. The activity of cholesteryl ester transfer protein in 44 non-obese subjects with impaired glucose tolerance (plasma triglycerides 1.56-/+0.64 mmol/L; high density lipoprotein (HDL) cholesterol 0.96-/+0.25 mmol/L; and plasma insulin 78-/+8 pmol/L) and in 42 normoglycemic controls (plasma triglycerides 0.88-/+0.41 mmol/L; HDL cholesterol 1.48-/+0.29 mmol/L; and plasma insulin 38-/+14 pmol/L) was measured with a new fluorometric assay.

RESULTS

Cholesteryl ester transfer protein activity was increased in subjects with impaired glucose tolerance by 47% (39.5-/+7.8 vs 26.8-/+6.8 nmol/mL x h-1; t-test, p<0.05). Linear regression analysis showed that cholesteryl ester transfer protein activity in subjects with impaired glucose tolerance significantly correlated with the following parameters: plasma triglycerides (r=0.614, p<0.05), HDL-triglycerides (r=0.595, p<0.05), percentage of HDL-triglyceride (r=0.667, p<0.05), percentage of HDL cholesterol ester (r=-0.751, p<0.01), percentage of HDL phospholipid (r=0.648, p<0.05), 2-h-insulin (r=0.668, p<0.05), and 2-h-proinsulin (r=0.658, p<0.01). In a subgroup of 13 subjects with impaired glucose tolerance, cholesteryl ester transfer protein activity correlated with HDL apoA-I fractional catabolic rate (r=0.701, p<0.01). In normoglycemic subjects, significant correlations were found only between cholesteryl ester transfer protein activity and percentage of HDL-triglycerides (r=0.541, p<0.05), percentage of HDL cholesteryl ester (r=-0.639, p<0.01), 2-h-proinsulin (r=0.642, p<0.05), and HDL apoA-I fractional catabolic rate (n=10, r=0.587, p<0.05).

CONCLUSION

Cholesteryl ester transfer is important for HDL composition and HDL catabolism both in normoglycemic subjects and subjects with impaired glucose tolerance. Under insulin resistant conditions, increased cholesteryl ester transfer protein activity modulates HDL metabolism more drastically than in normoglycemic conditions. This modulation may be explained by increased availability of triglyceride-rich lipoproteins for neutral lipid exchange in subjects with impaired glucose tolerance.

摘要

目的

研究糖耐量受损患者中胆固醇酯（中性脂质）转移蛋白活性的机制和动态变化。

方法

1998年1月至1999年12月期间，通过广告从当地人群中招募了86名白种人受试者。采用一种新的荧光测定法测量了44名糖耐量受损的非肥胖受试者（血浆甘油三酯1.56±0.64 mmol/L；高密度脂蛋白（HDL）胆固醇0.96±0.25 mmol/L；血浆胰岛素78±8 pmol/L）和42名血糖正常对照者（血浆甘油三酯0.88±0.41 mmol/L；HDL胆固醇1.48±0.29 mmol/L；血浆胰岛素38±14 pmol/L）的胆固醇酯转移蛋白活性。

结果

糖耐量受损患者的胆固醇酯转移蛋白活性增加了47%（39.5±7.8对26.8±6.8 nmol/mL·h-1；t检验，p<0.05）。线性回归分析显示，糖耐量受损患者的胆固醇酯转移蛋白活性与以下参数显著相关：血浆甘油三酯（r=0.614，p<0.05）、HDL-甘油三酯（r=0.595，p<0.05）、HDL-甘油三酯百分比（r=0.667，p<0.05）、HDL胆固醇酯百分比（r=-0.751，p<0.01）、HDL磷脂百分比（r=0.648，p<0.05）、2小时胰岛素（r=0.668，p<0.05）和2小时胰岛素原（r=0.658，p<0.01）。在13名糖耐量受损的受试者亚组中，胆固醇酯转移蛋白活性与HDL载脂蛋白A-I分数分解代谢率相关（r=0.701，p<0.01）。在血糖正常的受试者中，仅在胆固醇酯转移蛋白活性与HDL-甘油三酯百分比（r=0.541，p<0.05）、HDL胆固醇酯百分比（r=-0.639，p<0.01）、2小时胰岛素原（r=0.642，p<0.05）和HDL载脂蛋白A-I分数分解代谢率（n=10，r=0.587，p<0.05）之间发现显著相关性。