因子 XII 及其活性片段（β - XIIa）的分离、纯化与特性
[Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)].
作者信息
Blokhina T B, Neshkova E A, Iarovaia G A
机构信息
Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2, Moscow, 123995 Russia.
出版信息
Vopr Med Khim. 2002 Nov-Dec;48(6):603-10.
A procedure of isolation of human blood plasma prekallikrein, coagulation factor XII and active fragment beta-XIIa has been developed. This procedure includes the traditional chromatography steps and FPLC. Disc-electrophoresis revealed that the preparations of factor XII and beta-XIIa were homogeneous. Their specific activity was 70.6 U and 2.5 U, respectively. The procedure described is less time consuming and it allows to isolate these factors in the preparative quantities.
已开发出一种分离人血浆前激肽释放酶、凝血因子XII和活性片段β-XIIa的方法。该方法包括传统的色谱步骤和快速蛋白质液相色谱。圆盘电泳显示，因子XII和β-XIIa的制剂是均一的。它们的比活性分别为70.6 U和2.5 U。所描述的方法耗时较少，并且能够以制备量分离这些因子。
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