Schieferstein G
Fortschr Med. 1976 May 13;94(14):833-7.
The intraperitoneal diffusion-chamber-technique is a closed cell culture system in vivo. Hamster melanoma cells were capable in iso- and heterologous host animals for exponential proliferation. When the host animals had been preimmunized with melanoma cells or hamster lymphocytes a rapid cell lysis was observed in the chambers within 4 hrs after implantation. The cell numbers were reduced about 1-10% of the inoculum. After 6-10 days the cell numbers in chambers from immunized hosts slowly increased in spite of a high level of cytotoxicity in the serum of the animals.
腹腔扩散室技术是一种体内封闭细胞培养系统。仓鼠黑色素瘤细胞能够在同种和异种宿主动物体内进行指数增殖。当宿主动物预先用黑色素瘤细胞或仓鼠淋巴细胞免疫后，在植入后4小时内观察到扩散室内细胞迅速裂解。细胞数量减少至接种量的约1 - 10%。尽管动物血清中细胞毒性水平很高，但在免疫宿主的扩散室中，6 - 10天后细胞数量仍缓慢增加。
