Michalek S M, McGhee J R, Navia J M, Narkates A J
Infect Immun. 1976 Mar;13(3):782-9. doi: 10.1128/iai.13.3.782-789.1976.
When rats from dams fed a low-protein diet were injected with whole, killed Strepococcus mutans 6715 cells in the region of the submandibular gland, they developed serum and salivary agglutinins to this microorganism. Titers of agglutinins in malnourished rats were similar to those observed in rats from dams fed a nutritionally adequate diet that were locally injected with S. mutans. Furthermore, both groups of immunized rats subsequently infected with cariogenic S. mutans 6715 had lower mean caries scores than infected, nonimmunized rats. This reduced incidence of caries scores than infected, nonimmunized rats. This reduced incidence of caries was evident on all molar surfaces. The mean body weights of immunized and nonimmunized, protein-deficient rats were not significantly different; likewise, both immunized and nonimmunize normally nourished rats exhibited similar weight gains. Malnourished rats, not immunized but infected with S. mutans, had significantly more caries than normal, nonimmunized infected rats. Both dietary groups of noninfected rats had very few carious lesions. These results suggest that carious lesions observed in these rats resulted from S. mutans 6715 infection. Furthermore, protein-malnourished rats, injected in the region of the submandibular gland with whole, killed S. mutans elicit an immune response and are protected against S. mutans-induced caries.
当给喂食低蛋白饮食的母鼠所产的大鼠在下颌下腺区域注射完整的、灭活的变形链球菌6715细胞时，它们产生了针对这种微生物的血清和唾液凝集素。营养不良大鼠体内的凝集素滴度与给喂食营养充足饮食的母鼠所产且局部注射变形链球菌的大鼠中观察到的滴度相似。此外，随后感染致龋变形链球菌6715的两组免疫大鼠的平均龋齿评分均低于未免疫的感染大鼠。与未免疫的感染大鼠相比，这种龋齿发病率的降低很明显。在所有磨牙表面，龋齿发病率的降低都很明显。免疫的和未免疫的蛋白质缺乏大鼠的平均体重没有显著差异；同样，免疫的和未免疫的正常营养大鼠体重增加情况相似。未免疫但感染了变形链球菌的营养不良大鼠的龋齿明显多于正常的未免疫感染大鼠。两组未感染大鼠的饮食组都很少有龋损。这些结果表明，在这些大鼠中观察到的龋损是由变形链球菌6715感染引起的。此外，在下颌下腺区域注射完整的、灭活的变形链球菌的蛋白质营养不良大鼠会引发免疫反应，并受到保护，防止变形链球菌诱导的龋齿。
