GSH2，一种编码多形汉逊酵母中γ-谷氨酰半胱氨酸合成酶的基因。

GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha.

作者信息

Ubiyvovk Vira M, Nazarko Taras Y, Stasyk Olena G, Sohn Min Jeong, Kang Hyun Ah, Sibirny Andrei A

机构信息

Institute of Cell Biology, Drahomanov Str. 14/16, Lviv 79005, Ukraine.

出版信息

FEMS Yeast Res. 2002 Aug;2(3):327-32. doi: 10.1016/S1567-1356(02)00111-3.

DOI:10.1016/S1567-1356(02)00111-3

PMID:12702282

Abstract

The GSH2 gene, encoding Hansenula polymorpha gamma-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.

摘要

编码多形汉逊酵母γ-谷氨酰半胱氨酸合成酶的GSH2基因，是通过对多形汉逊酵母谷胱甘肽（GSH）缺陷型gsh2突变体进行功能互补而克隆得到的。该基因作为一个4.3kb的XbaI片段被分离出来，当导入gsh2突变体细胞时，它能够恢复GSH合成、重金属抗性和细胞增殖。与白色念珠菌同源物（Gcs1p）相比，它具有53%的相同氨基酸和69%的相似氨基酸。与酿酒酵母同源物（Gsh1p）相比，它具有47%的相同氨基酸和61%的相似氨基酸。GSH2序列出现在GenBank数据库中，登录号为AF435121。