单个温度敏感突变对大肠杆菌全局基因表达的影响。
The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli.
作者信息
Li Yong, Cole Kyle, Altman Sidney
机构信息
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut 06520, USA.
出版信息
RNA. 2003 May;9(5):518-32. doi: 10.1261/rna.2198203.
Abstract
High-density DNA microarrays have been used to explore the genomic profiling of gene expression of a defective Escherichia coli strain with a temperature-sensitive mutation in the protein component of RNase P. A novel gene cluster was discovered in which two of the genes are known substrates of RNase P. The expression pattern of essential genes and gene discovery from intergenic regions, for which other new transcripts are found, are also discussed.
摘要
高密度DNA微阵列已被用于探索一种有缺陷的大肠杆菌菌株的基因表达基因组谱，该菌株在核糖核酸酶P的蛋白质成分中存在温度敏感突变。发现了一个新的基因簇，其中两个基因是核糖核酸酶P的已知底物。还讨论了必需基因的表达模式以及从基因间区域发现的基因，在这些区域发现了其他新的转录本。
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