Ren Ying, Kruhlak Michael J, Bazett-Jones David P
Programme in Cell Biology, The Hospital for Sick Children, Toronto, Ontario, Canada.
J Histochem Cytochem. 2003 May;51(5):605-12. doi: 10.1177/002215540305100506.
Correlative imaging of a specific cell with both the light microscope and the electron microscope has proved to be a difficult task, requiring enormous amounts of patience and technical skill. We describe a technique with a high rate of success, which can be used to identify a particular cell in the light microscope and then to embed and thin-section it for electron microscopy. The technique also includes a method to obtain many uninterrupted, thin serial sections for imaging by conventional or energy-filtered transmission electron microscopy, to obtain images for 3D analysis of detail at the suborganelle level.
事实证明，利用光学显微镜和电子显微镜对特定细胞进行相关成像，是一项艰巨的任务，需要极大的耐心和专业技术。我们描述了一种成功率很高的技术，该技术可用于在光学显微镜下识别特定细胞，然后将其包埋并制成超薄切片用于电子显微镜观察。该技术还包括一种方法，可获得许多连续的超薄切片，用于传统或能量过滤透射电子显微镜成像，以获取亚细胞器水平细节的三维分析图像。
