Kudo Yoshinori, Minegishi Masayoshi, Saito Naoko, Itoh Tsuneo, Fushimi Junko, Takahashi Hiroyuki, Suzuki Akira, Narita Ayuko, Sato Yuko, Miura Junko, Yoshihara Kazue, Meguro Kuniaki, Imaizumi Masue, Sato Makoto, Murakawa Yasuko, Tsuchiya Shigeru
Division of Blood Transfusion, Tohoku University Hospital, Sendai 980-8574, Japan.
Tohoku J Exp Med. 2003 Feb;199(2):111-8. doi: 10.1620/tjem.199.111.
Retrospective analysis was conducted in 51 autologous peripheral blood progenitor cell (PBPC) collections using the Spectra AutoPBSC System from patients with hematologic malignancies and solid tumors to study the predictive value of CD34+ cell counts in the peripheral blood for the yield of CD34+ cells in the apheresis product. The correlation coefficients for CD34+ cells microL(-1) of peripheral blood with CD34+ cell yield (x 10(6) kg(-1) of body weight and x 10(5) kg(-1) of body weight L(-1) of blood processed) were 0.903 and 0.778 (n=51 collections), respectively. Products collected from patients with CD34+ cell counts below 15 microL(-1) in the peripheral blood contained a median of 0.49 x 10(6) CD34+ cells kg(-1) (range: 0.05-2.55), whereas those with CD34+ cell counts more than 15 microL(-1) contained a median of 3.72 x 10(6) CD34+ cells kg(-1) (range: 1.06-37.57). From these results, a number of at least 15 CD34+ cells microL(-1) in the peripheral blood ensured a minimum yield of 1 x 10(6) CD34+ cells kg(-1) as obtained by a single apheresis procedure. The number of CD34+ cells in the peripheral blood can be used as a good predictor for timing of apheresis and estimating PBPC yield. With regard to our results, apheresis with a possibly poor efficiency should be avoided because the collection procedure is time-consuming and expensive.
采用Spectra自动外周血祖细胞采集系统，对51例血液系统恶性肿瘤和实体瘤患者的自体外周血祖细胞（PBPC）采集进行回顾性分析，以研究外周血中CD34+细胞计数对单采产品中CD34+细胞产量的预测价值。外周血中每微升CD34+细胞与CD34+细胞产量（每千克体重×10⁶个及每千克体重每升处理血液×10⁵个）的相关系数分别为0.903和0.778（n = 51次采集）。外周血中CD34+细胞计数低于15/μL的患者所采集的产品中，CD34+细胞中位数为0.49×10⁶/kg（范围：0.05 - 2.55），而CD34+细胞计数高于15/μL的患者所采集的产品中，CD34+细胞中位数为3.72×10⁶/kg（范围：1.06 - 37.57）。根据这些结果，外周血中至少15个/μL的CD34+细胞可确保单次单采程序获得的CD34+细胞最低产量为1×10⁶/kg。外周血中CD34+细胞数量可作为单采时机和估算PBPC产量的良好预测指标。基于我们的研究结果，应避免效率可能较低的单采，因为采集过程既耗时又昂贵。
