Maeda M, Tsuji A
J Chromatogr. 1976 May 26;120(2):449-55. doi: 10.1016/s0021-9673(76)80021-0.
Methods for determining benomyl [methyl 1-(butylcarbamoyl)-2-benzimidazole carbamate] residues in plant tissues involve isolation of the residue by extraction with ethyl acetate, hydrolysis of the extracted residue to 2-aminobenzimidazole and final determination by high-performance liquid chromatography using a fluorimeter. 2-(4-Thiazolyl) benzimidazole is also determined by a similar procedure without hydrolysis. The sensitivities of these methods are 0.02 ppm for benomyl and 0.001 ppm for 2-(4-thiazolyl) benzimidazole. Recoveries of these compounds from various plant tissues were 90.5-102.9%. No interference with these methods from the plant tissues tested was found.
测定植物组织中苯菌灵（甲基 1-(丁基氨基甲酰基)-2-苯并咪唑氨基甲酸酯）残留量的方法包括用乙酸乙酯萃取分离残留量，将萃取的残留量水解为 2-氨基苯并咪唑，最后使用荧光计通过高效液相色谱法进行测定。2-(4-噻唑基)苯并咪唑也通过类似程序测定，但无需水解。这些方法的灵敏度对于苯菌灵为 0.02 ppm，对于 2-(4-噻唑基)苯并咪唑为 0.001 ppm。这些化合物从各种植物组织中的回收率为 90.5 - 102.9%。未发现所测试的植物组织对这些方法有干扰。
