Park C S, Chandler P T, Norman A W
J Dairy Sci. 1976 May;59(5):868-75. doi: 10.3168/jds.S0022-0302(76)84290-7.
To identify the limiting amino acid in the minimal essential medium as published by Eagle (Science 130:432, 1959) for milk protein synthesis in rat mammary cells in tissue culture, two different experimental approaches were used. The first study involved the reduction of amino acids singly from the total amino acid complement of the medium for milk protein synthesis. The second study was to investigate the effect on milk protein synthesis of single amino acid addition to the basic complement of amino acids. Order of limiting amino acids was lysine (first) and possible methionine, valine, or arginine (second).
为了确定伊格尔（《科学》130:432，1959年）发表的用于组织培养的大鼠乳腺细胞中乳蛋白合成的最低基本培养基中的限制氨基酸，采用了两种不同的实验方法。第一项研究涉及从用于乳蛋白合成的培养基的总氨基酸组成中逐一减少氨基酸。第二项研究是调查向基本氨基酸组成中单独添加氨基酸对乳蛋白合成的影响。限制氨基酸的顺序是赖氨酸（第一）和可能的蛋氨酸、缬氨酸或精氨酸（第二）。
