基于单光子计数的DNA测序仪中荧光检测的动态范围和碱基识别准确性。
Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting.
作者信息
Gavrilov Dmitri N, Gorbovitski Boris, Gouzman Mikhail, Gudkov Georgiy, Stepoukhovitch Andrei, Ruskovoloshin Victor, Tsuprik Andrei, Tyshko Georgiy, Bilenko Olga, Kosobokova Olga, Luryi Serge, Gorfinkel Vera
机构信息
Department of Electrical and Computer Engineering, State University of New York, Stony Brook 11794, USA.
出版信息
Electrophoresis. 2003 Apr;24(7-8):1184-92. doi: 10.1002/elps.200390152.
Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy.
最近，我们基于对荧光标记DNA片段的单光子检测开发了一系列高性能自动化毛细管DNA测序仪。我们的仪器采用了数字和宽带技术，这对于实现卓越的仪器灵敏度和动态范围至关重要。在本文中，我们讨论了由单光子探测器的非线性导致的仪器性能限制，以及用于增加检测动态范围和碱基识别准确性的非线性补偿方法。
Zotero 插件