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一种在葡萄糖存在下测定右旋糖酐的半自动微量法。

A semiautomated micromethod for dextran in the presence of glucose.

作者信息

Weet J F, Cobb C A, Lebrie S J

出版信息

J Lab Clin Med. 1976 May;87(5):898-902.

PMID:1270896
Abstract

A semiautomated method for microanalysis of dextran with anthrone reagent has been developed. The method utilizes the Technicon inulin manifold and other standard AutoAnalyzer equipment. Since the anthrone method is sensitive to plasma glucose, a glocuse oxidase method which eliminates glucose in samples to be analyzed for dextran has also been developed. When solutions containing 50 mg. per 100 ml. of dextran were compared to solutions containing 50 mg. per 100 ml. of dextran plus up to 200 mg. per 100 ml. glucose, no significant difference was observed. The coefficient of the anthrone-glucose oxidase method is 0.039, which is within the error of the AutoAnalyzer itself.

摘要

已开发出一种用蒽酮试剂对葡聚糖进行微量分析的半自动方法。该方法使用Technicon菊粉流路及其他标准自动分析仪设备。由于蒽酮法对血浆葡萄糖敏感,还开发了一种葡萄糖氧化酶法,可在分析葡聚糖的样品中消除葡萄糖。当将每100毫升含50毫克葡聚糖的溶液与每100毫升含50毫克葡聚糖加高达每100毫升200毫克葡萄糖的溶液进行比较时,未观察到显著差异。蒽酮 - 葡萄糖氧化酶法的系数为0.039,这在自动分析仪本身的误差范围内。

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1
A semiautomated micromethod for dextran in the presence of glucose.一种在葡萄糖存在下测定右旋糖酐的半自动微量法。
J Lab Clin Med. 1976 May;87(5):898-902.
2
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