Rautava J, Soukka T, Heikinheimo K, Miettinen P J, Happonen R-P, Jaakkola P
Department of Oral and Maxillofacial Surgery, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, Finland.
J Dent Res. 2003 May;82(5):382-7. doi: 10.1177/154405910308200511.
Syndecan-1 expression is enhanced in cutaneous and mucosal wounds. We have previously demonstrated that wounding-induced syndecan-1 expression in the skin occurs transcriptionally, through a fibroblast-growth-factor-inducible element (FiRE). Here, we show that FiRE is also activated in mucosal wounds. However, both the expression patterns and the activation mechanisms of FiRE are different from those in the skin. In the mucosa in vivo, the activation starts and ends earlier than in cutaneous wounds. FiRE is first detected at around 12 hours in keratinocytes, and the activation declines by the third day after wounding occurs. The activation is seen on the migrating sheet of epithelial mucosa, as in the case of cutaneous wounding. In contrast to the situation in vivo, organ-cultured mucosal wounds exhibit no FiRE activity, while organ-cultured cutaneous wounds show robust activity. Activation in mucosal wounds is enhanced, however, by the application of epidermal growth factor. This suggests that exogenous growth factor activity is required for activation of syndecan-1 in mucosal wounds but not in cutaneous wounds.
Syndecan-1在皮肤和黏膜伤口中表达增强。我们之前已经证明，皮肤中创伤诱导的syndecan-1表达是通过成纤维细胞生长因子诱导元件（FiRE）在转录水平上发生的。在此，我们表明FiRE在黏膜伤口中也被激活。然而，FiRE的表达模式和激活机制与皮肤中的不同。在体内黏膜中，激活比皮肤伤口开始得早且结束得也早。FiRE最早在角质形成细胞中于约12小时被检测到，并且在创伤发生后第三天激活下降。与皮肤创伤一样，在迁移的上皮黏膜片上可见激活。与体内情况相反，器官培养的黏膜伤口未表现出FiRE活性，而器官培养的皮肤伤口显示出强大的活性。然而，通过应用表皮生长因子可增强黏膜伤口中的激活。这表明外源性生长因子活性是黏膜伤口中syndecan-1激活所必需的，但在皮肤伤口中并非如此。
