Pollard D R, Baran M M, Bachvarova R
J Embryol Exp Morphol. 1976 Feb;35(1):169-78.
Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from 0-01 to 1-0 mug/ml in vitro for two days from the 8-cell stage exhibit a concentration-dependent decrease in the frequency of normal blastocysts and decrease in average cell number per embryo. A 20-h exposure was adequate to achieve the full BUdR response. Both effects were eliminated in the presence of excess thymidine. Autoradiographs demonstrated that BUdR[3H] was incorporated into DNA during the first and second day of culture. Thus, BUdR appears to act through incorporation into DNA; and, in this system, cell division is at least as sensitive to BUdR as is differentiation.
从8细胞期开始，在体外将小鼠胚胎暴露于浓度范围为0.01至1.0微克/毫升的5-溴脱氧尿苷（BUdR）中两天，正常囊胚的频率会出现浓度依赖性下降，且每个胚胎的平均细胞数也会减少。20小时的暴露足以实现完整的BUdR反应。在存在过量胸苷的情况下，这两种效应均被消除。放射自显影片显示，在培养的第一天和第二天，[3H]BUdR被掺入DNA中。因此，BUdR似乎是通过掺入DNA起作用的；并且，在这个系统中，细胞分裂对BUdR的敏感性至少与分化一样高。
