Pinheiro Maria Leticia B, Feres-Filho Eduardo J, Graves Dana T, Takiya Christina M, Elsas Maria Ignez G, Elsas Pedro Paulo, Luz Ricardo A
Graduate Periodontics, Faculdade de Odontologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
J Periodontol. 2003 Mar;74(3):323-8. doi: 10.1902/jop.2003.74.3.323.
Platelet-derived growth factor (PDGF) is a mitogen and chemoattractant for cells of mesenchymal origin. Over-expression of PDGF-B can promote formation of inflammatory lesions in the lungs of transgenic mice. Moreover, continuous exposure to PDGF inhibits collagen production by osteoblastic cells. Thus, the expression of mitogenic factors in an inflammatory context may limit the differentiated function of cells, and thereby limit repair following periodontal attachment and bone loss. The goals of the present study were to test whether PDGF is present at increased levels in inflamed gingiva and to localize its expression in gingival biopsies from individuals with chronic periodontitis.
Tissues obtained during therapeutic procedures from inflamed and control sites of 9 patients were subjected to protein extraction, descriptive histology by hematoxylin and eosin, or immunohistochemistry assays. Quantification was calculated with an enzyme-linked immunosorbent assay (ELISA) kit specific for PDGF-AB. For the immunolocalization, anti-PDGF-A and -B antibodies were employed.
PDGF concentration in the total protein extract was approximately 3 times higher in the inflamed sites (0.60 +/- 0.18 ng/mg versus 0.20 +/- 0.05 ng/mg; P = 0.03). Immunohistochemistry revealed prominent expression of PDGF in the pocket epithelial cells as well as the adjacent connective tissue. In contrast, little or no expression was detected in control biopsies devoid of the pocket epithelium and granulation tissue.
PDGF is present in increased levels in the human inflamed gingiva and is mainly localized to the pocket epithelium. It is possible that chronic expression of PDGF contributes to the inflammatory changes that occur during periodontal diseases.
血小板衍生生长因子（PDGF）是一种对间充质来源细胞具有促有丝分裂作用和趋化作用的因子。PDGF - B的过度表达可促进转基因小鼠肺部炎症病变的形成。此外，持续暴露于PDGF会抑制成骨细胞产生胶原蛋白。因此，在炎症环境中促有丝分裂因子的表达可能会限制细胞的分化功能，从而限制牙周附着丧失和骨质流失后的修复。本研究的目的是检测PDGF在发炎牙龈中的水平是否升高，并将其表达定位在慢性牙周炎患者的牙龈活检组织中。
从9名患者的发炎部位和对照部位在治疗过程中获取的组织进行蛋白质提取、苏木精和伊红染色描述性组织学检查或免疫组织化学分析。使用针对PDGF - AB的酶联免疫吸附测定（ELISA）试剂盒进行定量计算。对于免疫定位，使用抗PDGF - A和 - B抗体。
发炎部位总蛋白提取物中的PDGF浓度约为对照部位的3倍（0.60±0.18 ng/mg对0.20±0.05 ng/mg；P = 0.03）免疫组织化学显示，PDGF在袋状上皮细胞以及相邻的结缔组织中显著表达。相比之下，在没有袋状上皮和肉芽组织的对照活检组织中几乎检测不到或未检测到表达。
PDGF在人类发炎牙龈中的水平升高，并且主要定位于袋状上皮。PDGF的慢性表达可能导致牙周疾病期间发生的炎症变化。
