Spruck Charles H, de Miguel Maria P, Smith Adrian P L, Ryan Aimee, Stein Paula, Schultz Richard M, Lincoln A Jeannine, Donovan Peter J, Reed Steven I
Department of Molecular Biology, MB-7, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Science. 2003 Apr 25;300(5619):647-50. doi: 10.1126/science.1084149.
We generated mice lacking Cks2, one of two mammalian homologs of the yeast Cdk1-binding proteins, Suc1 and Cks1, and found them to be viable but sterile in both sexes. Sterility is due to failure of both male and female germ cells to progress past the first meiotic metaphase. The chromosomal events up through the end of prophase I are normal in both CKS2-/- males and females, suggesting that the phenotype is due directly to failure to enter anaphase and not a consequence of a checkpoint-mediated metaphase I arrest.
我们培育出了缺乏Cks2的小鼠，Cks2是酵母Cdk1结合蛋白Suc1和Cks1的两个哺乳动物同源物之一，发现这些小鼠能够存活，但两性均不育。不育是由于雄性和雌性生殖细胞都无法越过减数第一次分裂中期。在CKS2基因敲除的雄性和雌性小鼠中，直到减数第一次分裂前期结束的染色体事件都是正常的，这表明该表型直接是由于无法进入后期，而不是检查点介导的减数第一次分裂中期停滞的结果。
