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对在巴西亚马逊地区发现的棉鼠属(啮齿目:棉鼠科)物种进行的核型分析。

Karyotypic analysis in species of the genus Dasyprocta (Rodentia: Dasyproctidae) found in Brazilian Amazon.

作者信息

Ramos Rosemar S L, Vale William G, Assis Fátima L

机构信息

Department of Biology, CCB, UFPA, Belém, Pará, Brasil.

出版信息

An Acad Bras Cienc. 2003 Mar;75(1):55-69. doi: 10.1590/s0001-37652003000100007. Epub 2003 Apr 17.

DOI:10.1590/s0001-37652003000100007
PMID:12715050
Abstract

A total of 30 animals of the genus Dasyprocta were cytogenetically studied. They belong to the following species: D. prymnolopha (N=20), D. leporina (N=6), D. fuliginosa (N=1) and Dasyprocta sp. (N=3) (Dasyproctidae, Hystricognathi). Cell suspensions were obtained by peripheral blood culture, besides bone marrow and spleen cells, from D. prymnolopha and D. leporina. The diploid number was 64/65 for all samples. The karyotypes showed similarity, and chromosomal polymorphism was not detected by Giemsa conventional staining and G banding. The constitutive heterochromatin distribution at the pericentromeric region of all the chromosomes was similar in all species. D. prymnolopha, D. leporina and Dasyprocta sp. presented variation in the heterochromatical block size at one of the homologues of the A18 pair. D. fuliginosa presented the heterochromatin uniformly distributed in all chromosomes. There was not variation in the NORs pattern in the species studied.

摘要

总共对30只棉鼠属动物进行了细胞遗传学研究。它们属于以下物种:普氏棉鼠(N = 20)、棉鼠(N = 6)、黑棉鼠(N = 1)和棉鼠属某物种(N = 3)(棉鼠科,豪猪亚目)。除了骨髓和脾细胞外,还通过外周血培养从普氏棉鼠和棉鼠获得了细胞悬液。所有样本的二倍体数均为64/65。核型显示出相似性,吉姆萨常规染色和G带分析未检测到染色体多态性。所有物种中,所有染色体着丝粒周围区域的组成型异染色质分布相似。普氏棉鼠、棉鼠和棉鼠属某物种在A18对同源染色体之一的异染色质块大小上存在差异。黑棉鼠的异染色质在所有染色体中均匀分布。在所研究的物种中,核仁组织区(NORs)模式没有变化。

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