Herd J K, Hayhome B, Tschida J
Proc Soc Exp Biol Med. 1975 Oct;150(1):194-201. doi: 10.3181/00379727-150-39001.
Prolonged replacement of fetal calf serum by normal human serum for the enrichment of medium during tissue culture of Hurler fibroblasts resulted in increased acid mucopolysaccharides in the cells and in the medium. The predominant intracellular mucopolysaccharide had the characteristics of dermatan sulfate when Hurler cells were treated with either serum. Normal human serum contains a nonspecific coreective factor capable of augmenting the loss of 35SO4-AMPS from Hurler cells, but not from normal cells. Fetal calf serum and Hurler serum have similar corrective factor activity for labeled Hurler cells. The corrective factor activity of all three sera was recovered from reconstituted dialyzed ammonium sulfate precipitates. The corrective factor of normal human serum did not increase degradation of mucopolysaccharide, but increased secretion of macromolecular and large oligosaccharide components. Failure of the corrective factor of normal human serum to effectively decrease the dermatan sulfate content of Hurler cells during prolonged exposure may be a quantitative phenomenon due partly to the brief duration of corrective factor activity and partly to increased synthesis of mucopolysaccharide.
在对黏多糖贮积症Ⅰ型成纤维细胞进行组织培养时，用正常人血清长期替代胎牛血清以富集培养基，结果导致细胞和培养基中的酸性黏多糖增加。当用任何一种血清处理黏多糖贮积症Ⅰ型细胞时，细胞内主要的黏多糖具有硫酸皮肤素的特征。正常人血清含有一种非特异性矫正因子，它能够增加黏多糖贮积症Ⅰ型细胞中35SO4-AMPS的丢失，但对正常细胞无此作用。胎牛血清和黏多糖贮积症Ⅰ型血清对标记的黏多糖贮积症Ⅰ型细胞具有相似的矫正因子活性。所有三种血清的矫正因子活性均可从重构的透析硫酸铵沉淀物中恢复。正常人血清的矫正因子不会增加黏多糖的降解，但会增加大分子和大寡糖成分的分泌。正常人血清的矫正因子在长期暴露期间未能有效降低黏多糖贮积症Ⅰ型细胞中硫酸皮肤素的含量，这可能是一种定量现象，部分原因是矫正因子活性持续时间短暂，部分原因是黏多糖合成增加。
