Zhao Yong, Meng Xian-Min, Wei Ying-Jie, Zhao Xiu-Wen, Liu Dong-Qing, Cao Hui-Qing, Liew Choong-Chin, Ding Jin-Feng
Molecular Medicine Center for Cardiovascular Diseases, Cardiovascular Institute and Fu Wai Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, P.R. China.
J Mol Med (Berl). 2003 May;81(5):297-304. doi: 10.1007/s00109-003-0427-x. Epub 2003 Apr 30.
Cardiac-restricted genes play important roles in cardiovascular system. In an effort to identify such novel genes we identified a novel cardiac-specific kinase gene TNNI3K localized on 1p31.1 based on bioinformatics analyses. Sequence analysis suggested that TNNI3K is a distant family member of integrin-linked kinase. Northern blot and 76-tissue array analyses showed that TNNI3K is highly expressed in heart, but is undetectable in other tissues. Immunohistochemical analysis predominantly localized TNNI3K in the nucleus of cardiac myocytes. In vitro kinase assay showed that TNNI3K is a functional kinase. The yeast two-hybrid system showed that TNNI3K could directly interact with cardiac troponin I, results that were further confirmed by coimmunoprecipitation in vivo. Our data suggest that TNNI3K is a cardiac-specific kinase and play important roles in cardiac system.
心脏特异性基因在心血管系统中发挥着重要作用。为了鉴定此类新基因，我们基于生物信息学分析鉴定出一个位于1p31.1的新的心脏特异性激酶基因TNNI3K。序列分析表明TNNI3K是整合素连接激酶的远亲家族成员。Northern印迹和76组织阵列分析显示TNNI3K在心脏中高度表达，但在其他组织中无法检测到。免疫组织化学分析主要将TNNI3K定位在心肌细胞的细胞核中。体外激酶分析表明TNNI3K是一种功能性激酶。酵母双杂交系统显示TNNI3K可直接与心肌肌钙蛋白I相互作用，体内共免疫沉淀进一步证实了这一结果。我们的数据表明TNNI3K是一种心脏特异性激酶，在心脏系统中发挥重要作用。
