Honda A, Takahashi H, Toguri T, Ogawa T, Hase S, Ikegami M, Ehara Y
Department of Life Science, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Arch Virol. 2003 May;148(5):1017-26. doi: 10.1007/s00705-002-0959-3.
Tobacco plants expressing the mammalian 2'5'oligoadenylate system (2-5A system) exhibit resistance to cucumber mosaic virus (CMV). Here, to characterize the molecular aspect of the resistance to CMV in 2-5A system-expressing tobaccos, the activation of defense-related genes and systemic acquired resistance (SAR) as the markers for the hypersensitive resistance (HR), were elucidated. Northern hybridization analysis indicated that the expression of four pathogenesis-related (PR) protein genes and five HR-related genes were induced in CMV-infected tobaccos expressing 2-5A system. Furthermore, the induction of SAR against Pseudomonas syringae pv. tabaci as second challenge, was observed on CMV-inoculated tobaccos expressing 2-5A system. These results suggested that the resistance to CMV in tobacco expressing 2-5A system is associated with the establishment of an HR-like response.
表达哺乳动物2'5'寡腺苷酸系统（2-5A系统）的烟草植株对黄瓜花叶病毒（CMV）具有抗性。在此，为了表征表达2-5A系统的烟草对CMV抗性的分子层面，阐明了防御相关基因的激活以及作为超敏抗性（HR）标志物的系统获得性抗性（SAR）。Northern杂交分析表明，在感染CMV的表达2-5A系统的烟草中，四个病程相关（PR）蛋白基因和五个HR相关基因的表达被诱导。此外，在接种CMV的表达2-5A系统的烟草上观察到针对丁香假单胞菌烟草致病变种作为第二次挑战的SAR诱导。这些结果表明，表达2-5A系统的烟草对CMV的抗性与类HR反应的建立有关。
