Czyz W, Balcerczak E, Rudowicz M, Niewiadomska H, Pasieka Z, Kuzdak K, Mirowski M
Department of Endocrinological and General Surgery, Molecular Biology Laboratory, Medical University, Lódź, Poland.
Folia Histochem Cytobiol. 2003;41(2):91-5.
C-ERBB2 and P65 gene expression was investigated by reverse transcriptase polymerase chain reaction method (RT-PCR) in thirty follicular thyroid cancers and twenty follicular adenomas. Additionally, the cancers and adenomas were stained by immunohistochemistry for the expression of their protein products. We did not observe P65 gene expression in any of the analyzed follicular cancers (n=30) but it was observed in 13 out of 20 (65%) follicular adenomas. The presence of C-ERBB2 gene expression was found in 18 (90%) follicular adenomas but not in cancers. There were 10 (50%) adenomas and 11 (36.7%) cancers with positive staining for C-ERBB2 protein and 15 (75%) adenomas and 2 (6.7%) cancers with positive staining for P65 protein. We conclude that expression of C-ERBB2 and P65 genes is associated with follicular adenoma but not with cancer of thyroid gland.
采用逆转录聚合酶链反应法（RT-PCR）对30例滤泡状甲状腺癌和20例滤泡性腺瘤进行C-ERBB2和P65基因表达检测。此外，通过免疫组织化学对癌组织和腺瘤组织进行染色，以检测其蛋白产物的表达。在所分析的30例滤泡状癌中均未观察到P65基因表达，但在20例滤泡性腺瘤中有13例（65%）观察到P65基因表达。在18例（90%）滤泡性腺瘤中发现C-ERBB2基因表达，但在癌组织中未发现。C-ERBB2蛋白染色阳性的腺瘤有10例（50%），癌有11例（36.7%）；P65蛋白染色阳性的腺瘤有15例（75%），癌有2例（6.7%）。我们得出结论，C-ERBB2和P65基因的表达与滤泡性腺瘤相关，而与甲状腺癌无关。
