Garfias Yonathan, Rojas-Ramos Enrique, Jiménez María del Carmen, Martínez-Cairo Salvador, Chávez Raúl, Gorocica Patricia, Zenteno Edgar, Lascurain Ricardo
Departamento de Bioquímica, Instituto Nacional de Enfermedades Respiratorias, Tlalpan, México.
Immunol Invest. 2003 Feb;32(1-2):95-104. doi: 10.1081/imm-120019211.
Atopic disorders are driven by the Th2 cell subset. We have determined the expression of costimulatory molecules and cell surface markers on peripheral CD4+ T cells and antigen presenting cells, in different atopic diseases, and we have also tried to correlate the expression of these markers with the severity of the disease. Cells from patients with atopic and contact dermatitis, mild or severe asthma, and symptomatic and non-symptomatic atopic rhinitis were analyzed by flow cytometry. Our results showed that CD30, CD124, and CD152 expression on CD4+ T cells was significantly higher in atopic dermatitis than in contact dermatitis patients (p < 0.05). It was interesting to observe that the cell surface expression of CD80 in T and B cells from atopic dermatitis patients was not enhanced as opposed to the other atopic diseases we analyzed. Our results suggest that there are differences in the immune mechanisms involved in the different atopic diseases, and that expression of CD30 in CD4+ T cells might be a marker of disease activity in atopic dermatitis.
特应性疾病由Th2细胞亚群驱动。我们已经确定了不同特应性疾病中外周血CD4+ T细胞和抗原呈递细胞上共刺激分子和细胞表面标志物的表达情况，并且我们还试图将这些标志物的表达与疾病的严重程度相关联。通过流式细胞术分析了来自特应性皮炎和接触性皮炎患者、轻度或重度哮喘患者以及有症状和无症状特应性鼻炎患者的细胞。我们的结果显示，特应性皮炎患者CD4+ T细胞上CD30、CD124和CD152的表达显著高于接触性皮炎患者（p < 0.05）。有趣的是，与我们分析的其他特应性疾病不同，特应性皮炎患者T细胞和B细胞上CD80的细胞表面表达并未增强。我们的结果表明，不同特应性疾病所涉及的免疫机制存在差异，并且CD4+ T细胞中CD30的表达可能是特应性皮炎疾病活动的一个标志物。
