Goldenkova I V, Musiĭchuk K A, Piruzian E S
Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2003 Mar-Apr;37(2):356-64.
Bifunctional reporter proteins were constructed to combine Clostridium thermocellum lichenase (LicBM2) with Aequorea victoria green fluorescent protein (GFP) or with Escherichia coli beta-glucuronidase (GUS). The major properties of the initial proteins were preserved in the hybrid ones: LicBM2 was active at 65 degrees C, GFP fluoresced, and GUS hydrolyzed its substrates. LicBM2 remained active after extension of its C of N end. Bifunctional reporter systems were shown to provide a convenient tool for studying the gene expression regulation in prokaryotic (E. coli) and eukaryotic (Saccharomyces cerevisiae, mammalian) cells, advantages of one reporter compensating for drawbacks of the other.
构建了双功能报告蛋白，将嗜热栖热放线菌地衣酶（LicBM2）与维多利亚水母绿色荧光蛋白（GFP）或大肠杆菌β-葡萄糖醛酸酶（GUS）结合。初始蛋白的主要特性在杂合蛋白中得以保留：LicBM2在65摄氏度时具有活性，GFP能发出荧光，GUS能水解其底物。LicBM2在其C端或N端延长后仍保持活性。双功能报告系统被证明是研究原核细胞（大肠杆菌）和真核细胞（酿酒酵母、哺乳动物细胞）中基因表达调控的便捷工具，一种报告蛋白的优点可弥补另一种的缺点。
