[锌调节大鼠成骨细胞膜的Ca(2+)-ATP酶活性]

[Zinc modulates the Ca(2+)-ATPase activity of rat osteoblast membrane].

作者信息

Cen X, Wang R, Wang H

机构信息

Medical Experimental Center of First University Hospital, West China University of Medical Sciences, Chengdu 610041, China.

出版信息

Wei Sheng Yan Jiu. 2000 Jan 30;29(1):52-4.

PMID:12725045

Abstract

The effect and mechanism of zinc on Ca(2+)-ATPase and Na+, K(+)-ATPase activity of rat osteoblast membrane were studied. The activities were measured by colorimetric method. The effect of special inhibitors of protein kinase C or calmodulin on membrane Ca(2+)-ATPase and Na+, K(+)-ATPase activity which induced by zinc were also examined. The results showed that zinc could increase membrane Ca(2+)-ATPase activity, but no influence was observed on Na+, K(+)-ATPase activity. R24571, a special inhibitor of calmodulin, had no effect on membrane Ca(2+)-ATPase activity. Calmodulin also could not promote the basic Ca(2+)-ATPase activity of membrane. But trifluoperazine, a special inhibitor of protein kinase C, obviously reduced its activity. It was concluded that the modulation of zinc on membrane Ca(2+)-ATPase activity of rat osteoblast could be mediated by protein kinase C.

摘要

研究了锌对大鼠成骨细胞膜Ca(2+)-ATP酶和Na+、K(+)-ATP酶活性的影响及其机制。采用比色法测定酶活性。还检测了蛋白激酶C或钙调蛋白的特异性抑制剂对锌诱导的膜Ca(2+)-ATP酶和Na+、K(+)-ATP酶活性的影响。结果表明，锌可增加膜Ca(2+)-ATP酶活性，但对Na+、K(+)-ATP酶活性无影响。钙调蛋白特异性抑制剂R24571对膜Ca(2+)-ATP酶活性无影响。钙调蛋白也不能促进膜的基础Ca(2+)-ATP酶活性。但蛋白激酶C特异性抑制剂三氟拉嗪明显降低其活性。得出结论：锌对大鼠成骨细胞膜Ca(2+)-ATP酶活性的调节可能由蛋白激酶C介导。