Olejnik A, Grajek W, Marecik R
Department of Biotechnology and Food Microbiology, Agricultural University in Poznan, Wojska Polskiego 48 Street, Poznan, Poland.
J Biotechnol. 2003 May 8;102(3):291-300. doi: 10.1016/s0168-1656(03)00034-8.
A lot of strategies were applied to improve recombinant protein productivity in the baculovirus expression system. In this study we propose for foreign protein production fed-batch cultivation method at hyperosmotic environment induced by increased NaCl content. Obtained results suggested relatively high tolerance and adaptation abilities of Tn-5 insect cells exposed to hyperosmotic stress. The cells under hyperosmotic conditions increased the specific rate of glucose consumption and lactate production. The release of additional energy and precursors as a result of increased metabolism by osmotically stressed culture was involved in recombinant protein synthesis. Recombinant nucleoprotein productivity in nutritional feeding cultures exposed to hyperosmolarity was about 72% higher than that obtained in batch culture at physiological osmolarity, but 31% was a result of feeding and the rest 41% was a result of hyperosmolarity and increasing Na(+) concentration.
人们应用了许多策略来提高杆状病毒表达系统中重组蛋白的产量。在本研究中，我们提出了一种在通过增加氯化钠含量诱导的高渗环境下进行补料分批培养以生产外源蛋白的方法。所得结果表明，Tn-5昆虫细胞在高渗胁迫下具有较高的耐受性和适应能力。高渗条件下的细胞提高了葡萄糖消耗和乳酸产生的比速率。渗透应激培养物因代谢增加而释放的额外能量和前体参与了重组蛋白的合成。暴露于高渗环境的营养补料培养物中重组核蛋白的产量比生理渗透压下的分批培养高出约72%，但其中31%是补料的结果，其余41%是高渗和钠离子浓度增加的结果。
