一种对非洲爪蟾DNA复制起始至关重要的新型环状非洲爪蟾蛋白质复合物。
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.
作者信息
Kubota Yumiko, Takase Youhei, Komori Yasunori, Hashimoto Yoshitami, Arata Toshiaki, Kamimura Yoichiro, Araki Hiroyuki, Takisawa Haruhiko
机构信息
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043, Japan.
出版信息
Genes Dev. 2003 May 1;17(9):1141-52. doi: 10.1101/gad.1070003.
Abstract
We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.
摘要
我们已经鉴定出了芽殖酵母Sld5及其三种相互作用蛋白的非洲爪蟾同源物。这些蛋白形成了一种新型复合物，对非洲爪蟾卵提取物中DNA复制的起始至关重要。该复合物以依赖于复制许可和S期细胞周期蛋白依赖性激酶（S-phase CDK）的方式与染色质结合。该复合物与染色质的结合以及Cdc45与染色质的结合是相互依赖的，且这两种结合都需要非洲爪蟾Cut5，其酵母同源物与Sld5相互作用。在复制染色质上，该复合物与Cdc45和MCM相互作用，MCM是复制机器的假定组成部分。电子显微镜进一步揭示该复合物具有环状结构。这些结果表明该复合物在DNA复制的延伸阶段以及起始阶段都发挥着重要作用。
相似文献
1
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication.一种对非洲爪蟾DNA复制起始至关重要的新型环状非洲爪蟾蛋白质复合物。
Genes Dev. 2003 May 1;17(9):1141-52. doi: 10.1101/gad.1070003.
2
Xenopus Cut5 is essential for a CDK-dependent process in the initiation of DNA replication.非洲爪蟾Cut5对于DNA复制起始过程中依赖细胞周期蛋白依赖性激酶（CDK）的进程至关重要。
EMBO J. 2003 May 15;22(10):2526-35. doi: 10.1093/emboj/cdg238.
3
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.非洲爪蟾中，在S期细胞周期蛋白依赖性激酶的控制下，DNA聚合酶α在染色质上的加载依赖于Cdc45。
EMBO J. 1998 Oct 1;17(19):5699-707. doi: 10.1093/emboj/17.19.5699.
4
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.在裂殖酵母的DNA复制过程中，SpSld3是将SpCdc45装载到染色质上并维持其在染色质上所必需的。
Mol Biol Cell. 2002 May;13(5):1462-72. doi: 10.1091/mbc.02-01-0006.
5
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.GINS是一种在芽殖酵母中进行染色体DNA复制所需的新型多蛋白复合物。
Genes Dev. 2003 May 1;17(9):1153-65. doi: 10.1101/gad.1065903.
6
Role for a Xenopus Orc2-related protein in controlling DNA replication.非洲爪蟾Orc2相关蛋白在控制DNA复制中的作用。
Nature. 1996 Jan 25;379(6563):357-60. doi: 10.1038/379357a0.
7
Sld3, which interacts with Cdc45 (Sld4), functions for chromosomal DNA replication in Saccharomyces cerevisiae.Sld3与Cdc45（Sld4）相互作用，在酿酒酵母中参与染色体DNA复制过程。
EMBO J. 2001 Apr 17;20(8):2097-107. doi: 10.1093/emboj/20.8.2097.
8
Ordered assembly of Sld3, GINS and Cdc45 is distinctly regulated by DDK and CDK for activation of replication origins.Sld3、GINS和Cdc45的有序组装受DDK和CDK的明显调控，以激活复制起点。
EMBO J. 2006 Oct 4;25(19):4663-74. doi: 10.1038/sj.emboj.7601347. Epub 2006 Sep 21.
9
Schizosaccharomyces pombe replication protein Cdc45/Sna41 requires Hsk1/Cdc7 and Rad4/Cut5 for chromatin binding.粟酒裂殖酵母复制蛋白Cdc45/Sna41与染色质结合需要Hsk1/Cdc7和Rad4/Cut5。
Chromosoma. 2004 Sep;113(3):145-56. doi: 10.1007/s00412-004-0302-8. Epub 2004 Aug 3.
10
CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast.在芽殖酵母中，Sld2和Sld3的细胞周期蛋白依赖性激酶（CDK）磷酸化启动DNA复制。
Nature. 2007 Jan 18;445(7125):328-32. doi: 10.1038/nature05465. Epub 2006 Dec 13.
引用本文的文献
1
GINS1 facilitates the development of lung adenocarcinoma via Wnt/β-catenin activation.GINS1通过激活Wnt/β-连环蛋白促进肺腺癌的发展。
World J Surg Oncol. 2025 Apr 7;23(1):122. doi: 10.1186/s12957-025-03786-2.
2
Site directed mutagenesis reveals functional importance of conserved amino acid residues within the N-terminal domain of Dpb2 in budding yeast.定点诱变揭示了出芽酵母中Dpb2 N端结构域内保守氨基酸残基的功能重要性。
Arch Microbiol. 2024 Dec 17;207(1):14. doi: 10.1007/s00203-024-04214-x.
3
Assembly, Activation, and Helicase Actions of MCM2-7: Transition from Inactive MCM2-7 Double Hexamers to Active Replication Forks.MCM2-7的组装、激活及解旋酶作用：从无活性的MCM2-7双六聚体到活性复制叉的转变
Biology (Basel). 2024 Aug 17;13(8):629. doi: 10.3390/biology13080629.
4
Single-molecule imaging reveals the mechanism of bidirectional replication initiation in metazoa.单分子成像揭示了原生动物中双向复制起始的机制。
Cell. 2024 Jul 25;187(15):3992-4009.e25. doi: 10.1016/j.cell.2024.05.024. Epub 2024 Jun 11.
5
Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation.DONSON 在脊椎动物 DNA 复制起始过程中 CMG 解旋酶组装中的新作用。
EMBO J. 2023 Sep 4;42(17):e114131. doi: 10.15252/embj.2023114131. Epub 2023 Jul 17.
6
GINS2 Is Downregulated in Peripheral Blood of Patients with Intervertebral Disk Degeneration and Promotes Proliferation and Migration of Nucleus Pulposus Cells.GINS2 在椎间盘退变患者外周血中表达下调，并促进髓核细胞的增殖和迁移。
Comput Math Methods Med. 2022 Sep 1;2022:1986348. doi: 10.1155/2022/1986348. eCollection 2022.
7
GINS2 regulates the proliferation and apoptosis of colon cancer cells through PTP4A1.GINS2 通过 PTP4A1 调节结肠癌细胞的增殖和凋亡。
Mol Med Rep. 2022 Apr;25(4). doi: 10.3892/mmr.2022.12633. Epub 2022 Feb 9.
8
Bioinformatics Analysis Explores Potential Hub Genes in Nonalcoholic Fatty Liver Disease.生物信息学分析探索非酒精性脂肪性肝病中的潜在枢纽基因。
Front Genet. 2021 Oct 29;12:772487. doi: 10.3389/fgene.2021.772487. eCollection 2021.
9
High content genome-wide siRNA screen to investigate the coordination of cell size and RNA production.高通量全基因组 siRNA 筛选以研究细胞大小和 RNA 生成的协调。
Sci Data. 2021 Jun 28;8(1):162. doi: 10.1038/s41597-021-00944-5.
10
The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes.内 S 期检查点直接调控复制延伸，以维持停滞复制叉的完整性。
Proc Natl Acad Sci U S A. 2021 Jun 15;118(24). doi: 10.1073/pnas.2019183118.
本文引用的文献
1
Xenopus Cut5 is essential for a CDK-dependent process in the initiation of DNA replication.非洲爪蟾Cut5对于DNA复制起始过程中依赖细胞周期蛋白依赖性激酶（CDK）的进程至关重要。
EMBO J. 2003 May 15;22(10):2526-35. doi: 10.1093/emboj/cdg238.
2
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.GINS是一种在芽殖酵母中进行染色体DNA复制所需的新型多蛋白复合物。
Genes Dev. 2003 May 1;17(9):1153-65. doi: 10.1101/gad.1065903.
3
CDK- and Cdc45-dependent priming of the MCM complex on chromatin during S-phase in Xenopus egg extracts: possible activation of MCM helicase by association with Cdc45.非洲爪蟾卵提取物中，S期染色质上MCM复合物的CDK和Cdc45依赖性引发：MCM解旋酶与Cdc45结合可能的激活作用
Genes Cells. 2003 Feb;8(2):145-61. doi: 10.1046/j.1365-2443.2003.00621.x.
4
The Xenopus Xmus101 protein is required for the recruitment of Cdc45 to origins of DNA replication.非洲爪蟾Xmus101蛋白是将Cdc45招募到DNA复制起点所必需的。
J Cell Biol. 2002 Nov 25;159(4):541-7. doi: 10.1083/jcb.200207090. Epub 2002 Nov 18.
5
Control of DNA replication licensing in a cell cycle.细胞周期中DNA复制执照的调控
Genes Cells. 2002 Jun;7(6):523-34. doi: 10.1046/j.1365-2443.2002.00544.x.
6
DNA replication in eukaryotic cells.真核细胞中的DNA复制。
Annu Rev Biochem. 2002;71:333-74. doi: 10.1146/annurev.biochem.71.110601.135425. Epub 2001 Nov 9.
7
The DNA polymerase domain of pol(epsilon) is required for rapid, efficient, and highly accurate chromosomal DNA replication, telomere length maintenance, and normal cell senescence in Saccharomyces cerevisiae.在酿酒酵母中，聚合酶ε的DNA聚合酶结构域对于快速、高效且高度精确的染色体DNA复制、端粒长度维持以及正常细胞衰老来说是必需的。
J Biol Chem. 2002 Aug 2;277(31):28099-108. doi: 10.1074/jbc.M111573200. Epub 2002 May 15.
8
SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast.在裂殖酵母的DNA复制过程中，SpSld3是将SpCdc45装载到染色质上并维持其在染色质上所必需的。
Mol Biol Cell. 2002 May;13(5):1462-72. doi: 10.1091/mbc.02-01-0006.
9
CDK phosphorylation of Drc1 regulates DNA replication in fission yeast.Drc1的细胞周期蛋白依赖性激酶（CDK）磷酸化调控裂殖酵母中的DNA复制。
Curr Biol. 2002 Apr 2;12(7):599-605. doi: 10.1016/s0960-9822(02)00739-x.
10
Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45.非洲爪蟾的Mcm10在Mcm2 - 7之后与DNA复制起点结合，并刺激Cdc45与起点的结合。
Mol Cell. 2002 Feb;9(2):233-40. doi: 10.1016/s1097-2765(02)00456-2.
Zotero 插件