大肠杆菌的yggH基因编码一种tRNA（m7G46）甲基转移酶。
The yggH gene of Escherichia coli encodes a tRNA (m7G46) methyltransferase.
作者信息
De Bie Lara G S, Roovers Martine, Oudjama Yamina, Wattiez Ruddy, Tricot Catherine, Stalon Victor, Droogmans Louis, Bujnicki Janusz M
机构信息
Laboratoire de Microbiologie, Université Libre de Bruxelles, B-1070 Brussels, Belgium.
出版信息
J Bacteriol. 2003 May;185(10):3238-43. doi: 10.1128/JB.185.10.3238-3243.2003.
Abstract
We cloned, expressed, and purified the Escherichia coli YggH protein and show that it catalyzes the S-adenosyl-L-methionine-dependent formation of N(7)-methylguanosine at position 46 (m(7)G46) in tRNA. Additionally, we generated an E. coli strain with a disrupted yggH gene and show that the mutant strain lacks tRNA (m(7)G46) methyltransferase activity.
摘要
我们克隆、表达并纯化了大肠杆菌YggH蛋白，结果表明它催化了tRNA中第46位的N(7)-甲基鸟苷（m(7)G46）的S-腺苷-L-甲硫氨酸依赖性形成。此外，我们构建了一个yggH基因被破坏的大肠杆菌菌株，并表明该突变菌株缺乏tRNA（m(7)G46）甲基转移酶活性。
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