Martinez-Garcia Esteban, Tormo Antonio, Navarro-Lloréns Juana Maria
Departamento de Bioquimica y Biologia Molecular I, Facultad de Ciencias Biológicas, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Curr Microbiol. 2003 May;46(5):365-70. doi: 10.1007/s00284-002-3814-6.
The nucleotide sequence downstream from the rpoS gene in Enterobacter cloacae and Kluyvera cryocrescens contains the slyA-pad1-yclC genes. The DNA sequence of Enterobacter cloacae CETC960 shows a 2.6-kb insertion of unknown origin between rpoS and slyA. This 2.6-kb sequence has also been detected in species of Salmonella and in Pseudomonas aeruginosa, but not in the same location. This insertion has been detected in all the Enterobacter cloacae clinical strains studied although the size of the rpoS-yclC region was highly variable, possibly owing to the presence of insertions and/or deletions. The study of the rpoS-mutS region in other enterobacteria also showed variability in size. Our results support the idea of a variational hot spot in the rpoS-mutS region that could be related to pathogenesis and horizontal transfer.
阴沟肠杆菌和嗜冷克吕沃尔氏菌中rpoS基因下游的核苷酸序列包含slyA-pad1-yclC基因。阴沟肠杆菌CETC960的DNA序列显示在rpoS和slyA之间有一个来源不明的2.6 kb插入片段。该2.6 kb序列也在沙门氏菌属物种和铜绿假单胞菌中检测到，但位置不同。在所研究的所有阴沟肠杆菌临床菌株中均检测到了这种插入，尽管rpoS-yclC区域的大小高度可变，这可能是由于插入和/或缺失的存在。对其他肠杆菌中rpoS-mutS区域的研究也表明大小存在变异性。我们的结果支持rpoS-mutS区域存在一个可变热点的观点，该热点可能与发病机制和水平转移有关。
