Mazancová Karla, Miksík Ivan, Kunes Jaroslav, Zicha Josef, Pácha Jirí
Institute of Physiology, Czech Academy of Sciences, Prague, Czech Republic.
Hypertens Res. 2003 Apr;26(4):333-8. doi: 10.1291/hypres.26.333.
To evaluate the role of sexually dimorphic tissue expression of 11beta-oxidase activity of 11beta-hydroxysteroid dehydrogenase (11betaHSD) in gender-associated blood pressure differences, we have studied female and male hypertensive rats of two different strains and their normotensive controls: spontaneously hypertensive rats (SHR), Wistar-Kyoto rats (WKY) and Dahl salt-sensitive (SS/Jr) and salt-resistant rats (SR/Jr). In hypertensive SHR and SS/Jr, but not in normotensive strains WKY and SR/Jr, blood pressure reached a higher level in males than in females. The activity of 11betaHSD was higher in the renal cortex, medulla, colon and aorta of males than of females in all investigated strains with the exception of aortic 11betaHSD in SHR and WKY rats, both of which had very low 11beta-oxidase activity. In contrast to gender-dependent differences, strain differences of 11betaHSD were observed in a limited number of tissues only. Renal medullary 11betaHSD showed significantly lower activity in WKY than in SHR, whereas no difference was observed in the renal cortex. Similarly, colonic 11betaHSD activity was lower in WKY than in SHR. In Dahl rats the strain differences were observed in aortic 11betaHSD that had higher activity in SR/Jr than in SS/Jr rats; no difference was observed in the kidney or colon. These data demonstrate the following. 1) Sexual dimorphism of 11betaHSD activity exists in the kidney, colon, and aorta. 2) The sexual dimorphism of 11betaHSD does not play a role in gender-associated differences in blood pressure. 3) The reduced 11betaHSD activity in the aorta of hypertensive SS/Jr compared to SR/Jr rats suggests that this enzyme might play a role in the pathogenesis of salt-sensitive hypertension in Dahl rats.
为评估11β - 羟类固醇脱氢酶（11βHSD）的11β - 氧化酶活性的性别二态性组织表达在性别相关血压差异中的作用，我们研究了两种不同品系的雌性和雄性高血压大鼠及其正常血压对照：自发性高血压大鼠（SHR）、Wistar - Kyoto大鼠（WKY）以及Dahl盐敏感大鼠（SS/Jr）和盐抵抗大鼠（SR/Jr）。在高血压的SHR和SS/Jr大鼠中，而非正常血压品系WKY和SR/Jr大鼠中，雄性大鼠的血压水平高于雌性。在所有研究的品系中，除了SHR和WKY大鼠主动脉中的11βHSD（二者的11β - 氧化酶活性都非常低）外，雄性大鼠肾皮质、髓质、结肠和主动脉中11βHSD的活性均高于雌性。与性别依赖性差异相反，仅在少数组织中观察到11βHSD的品系差异。肾髓质11βHSD在WKY大鼠中的活性显著低于SHR大鼠，而在肾皮质中未观察到差异。同样，结肠11βHSD活性在WKY大鼠中低于SHR大鼠。在Dahl大鼠中，主动脉11βHSD存在品系差异，SR/Jr大鼠中的活性高于SS/Jr大鼠；在肾脏或结肠中未观察到差异。这些数据表明：1）11βHSD活性在肾脏、结肠和主动脉中存在性别二态性。2）11βHSD的性别二态性在性别相关的血压差异中不起作用。3）与SR/Jr大鼠相比，高血压SS/Jr大鼠主动脉中11βHSD活性降低，提示该酶可能在Dahl大鼠盐敏感性高血压的发病机制中起作用。
