Renneberg Dorte, Dervan Peter B
Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
J Am Chem Soc. 2003 May 14;125(19):5707-16. doi: 10.1021/ja0300158.
The DNA binding properties of fused heterocycles imidazo[4,5-b]pyridine (Ip) and hydroxybenzimidazole (Hz) paired with pyrrole (Py) in eight-ring hairpin polyamides are reported. The recognition profile of Ip/Py and Hz/Py pairs were compared to the five-membered ring pairs Im/Py and Hp/Py on a DNA restriction fragment at four 6-base pair recognition sites which vary at a single position 5'-TGTNTA-3', where N = G, C, T, A. The Ip/Py pair distinguishes G.C from C.G, T.A, and A.T, and the Hz/Py pair distinguishes T.A from A.T, G.C, and C.G, affording a new set of heterocycle pairs to target the four Watson-Crick base pairs in the minor groove of DNA.
报道了八环发夹型聚酰胺中咪唑并[4,5 - b]吡啶（Ip）和羟基苯并咪唑（Hz）与吡咯（Py）融合的杂环的DNA结合特性。在四个6碱基对识别位点的DNA限制片段上，将Ip/Py和Hz/Py对的识别谱与五元环对Im/Py和Hp/Py进行了比较，这些识别位点在单个位置5'-TGTNTA-3'处有所不同，其中N = G、C、T、A。Ip/Py对可区分G.C与C.G、T.A和A.T，Hz/Py对可区分T.A与A.T、G.C和C.G，从而提供了一组新的杂环对，用于靶向DNA小沟中的四个沃森-克里克碱基对。
