处理乳酸克鲁维酵母β-半乳糖苷酶纯化的不同方法。
Dealing with different methods for Kluyveromyces lactis beta-galactosidase purification.
作者信息
Becerra M, Cerdãn E, Gonzãlez Siso M I
机构信息
Departamento de Bioloxía Celular e Molecular. Facultade de Ciencias. Campus da Zapateira s/n. 15071-A Coruña. Spain.
出版信息
Biol Proced Online. 1998 May 14;1:48-58. doi: 10.1251/bpo4.
Abstract
Several micro-scale chromatography-based procedures for purification of the beta-galactosidase from the yeast Kluyveromyces lactis were assayed. Purified enzyme was suitable to be used as antigen to induce polyclonal antibodies production. Specific staining of non-denaturing PAGE gels with chromogenic substrates allowed the determination of the number of subunits forming the native enzyme.
摘要
我们对几种基于微尺度色谱法从乳酸克鲁维酵母中纯化β-半乳糖苷酶的方法进行了测定。纯化后的酶适合用作抗原以诱导多克隆抗体的产生。使用显色底物对非变性聚丙烯酰胺凝胶进行特异性染色，可以确定构成天然酶的亚基数量。
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