Sjödahl R, Tagesson C
Scand J Gastroenterol. 1976;11(3):321-8.
Human gallbladder epithelium was homogenized with a view to maintaining the integrity of subcellular components. In such homogenates, N-acetyl-beta-glucosaminidase, beta-glucuronidase, beta-galactosidase, beta-glucosidase, beta-fucosidase, beta-xylosidase, and acid phosphatase were demonstrated together with phospholipase activity. All the enzymes exhibited structure-linked latency. After discarding cellular debris from the homogenate, remaining subcellular organelles were analytically separated by density gradient centrifugation. After 100,000 g for 1 hour, particles containing acid glycosidases were recovered at a sucrose density of 1.18-1.19, whereas the mitochondrial marker enzyme succinate-reductase accumulated at a density of 1.16. The bulk of sedimentable phospholipase activity was recovered with particles sedimenting at 1.18-1.19. The results are interpreted as indicating that phosphalipase is present in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, in lysosomes of the human gallbladder epithelium. Release of acid hydrolases, particularly phospholipase A, from the gallbladder epithelium is discussed as mediation of an inflammatory reaction in the gallbladder, i.e. cholecystitis.
为保持亚细胞成分的完整性，对人胆囊上皮进行了匀浆处理。在此类匀浆中，已证实存在N - 乙酰 - β - 氨基葡萄糖苷酶、β - 葡萄糖醛酸酶、β - 半乳糖苷酶、β - 葡萄糖苷酶、β - 岩藻糖苷酶、β - 木糖苷酶和酸性磷酸酶以及磷脂酶活性。所有这些酶均表现出与结构相关的潜伏性。从匀浆中去除细胞碎片后，通过密度梯度离心对剩余的亚细胞器进行分析分离。在100,000 g离心1小时后，含酸性糖苷酶的颗粒在蔗糖密度为1.18 - 1.19时被回收，而线粒体标记酶琥珀酸还原酶则在密度为1.16时聚集。大部分可沉降的磷脂酶活性与在1.18 - 1.19沉降的颗粒一起被回收。这些结果被解释为表明磷脂酶存在于人胆囊上皮的溶酶体中。人胆囊上皮溶酶体中酸性水解酶的释放。人胆囊上皮溶酶体中酸性水解酶的释放。人胆囊上皮溶酶体中酸性水解酶的释放。胆囊上皮中酸性水解酶，特别是磷脂酶A的释放被认为是胆囊炎症反应（即胆囊炎）的介导因素。
