Lian Hong-Zhen, Kang Yu-Fen, Yasin Arkin, Bi Shu-Ping, Shao Da-Lin, Chen Yi-Jun, Dai Le-Mei, Tian Li-Ching
State Key Laboratory of Coordination Chemistry, Department of Chemistry and Center of Materials Analysis, Nanjing University, 22 Hankou Road, Nanjing 210093, China.
J Chromatogr A. 2003 Apr 18;993(1-2):179-85. doi: 10.1016/s0021-9673(03)00359-5.
Morin was used as a pre-column reagent for the determination of aluminum by RP-HPLC with fluorescence detection. This method has been successfully applied to direct determination of trace Al in environmental and biological samples. The response was linear from 6 x 10(-9) to 6 x 10(-5) M with a detection limit of 2 x 10(-9) M. In addition, the different Al complexes with morin were separated by the proposed HPLC procedure and their coordination ratios were depicted by molar-ratio method. The results showed that 1:1 and 2:1 Al-morin complexes formed.
桑色素用作反相高效液相色谱荧光检测法测定铝的柱前试剂。该方法已成功应用于环境和生物样品中痕量铝的直接测定。响应在6×10⁻⁹至6×10⁻⁵ M范围内呈线性，检测限为2×10⁻⁹ M。此外，通过所提出的高效液相色谱法分离了与桑色素形成的不同铝配合物，并采用摩尔比法描述了它们的配位比。结果表明形成了1:1和2:1的铝 - 桑色素配合物。
