Chandel B S, Chauhan H C, Kher H N
Department of Microbiology, College of Veterinary Science and Animal Husbandry, Gujarat Agricultural University, Sardar Krushinagar, India.
Trop Anim Health Prod. 2003 Apr;35(2):99-104. doi: 10.1023/a:1022896117122.
The performance of the standard agar gel immunodiffusion (AGID) test and the competitive enzyme-linked immunosorbent assay (cELISA) for the detection of serum antibody against bluetongue virus (BTV) in clinically healthy and diseased camels in Gujarat state were compared. Out of 176 sera tested, 22 (12.5%) and 34 (19.3%) were positive for group-specific bluetongue antibodies by AGID and cELISA, respectively. Maximum seropositivities of 18.0% by AGID and 25.8% by cELISA were recorded in the Kutchhi breed, and of 6.9% and 12.6%, respectively, in the Marwari breed. The seroprevalence detected by AGID and cELISA in clinically healthy and diseased camels did not differ significantly with regard to bluetongue disease in these breeds.
比较了标准琼脂凝胶免疫扩散（AGID）试验和竞争性酶联免疫吸附测定（cELISA）在古吉拉特邦临床健康和患病骆驼中检测抗蓝舌病病毒（BTV）血清抗体的性能。在检测的176份血清中，分别有22份（12.5%）和34份（19.3%）通过AGID和cELISA检测出蓝舌病组特异性抗体呈阳性。库奇品种中AGID和cELISA的最高血清阳性率分别为18.0%和25.8%，马尔瓦里品种中分别为6.9%和12.6%。在这些品种中，AGID和cELISA在临床健康和患病骆驼中检测到的蓝舌病血清流行率没有显著差异。
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