Patton J F, Work T M, Jessup D A, Hietala S K, Oliver M N, Maclachlan N J
Department of Veterinary Pathology, School of Veterinary Medicine, University of California, Davis 95616.
J Wildl Dis. 1994 Jan;30(1):99-102. doi: 10.7589/0090-3558-30.1.99.
Three adult black-tailed deer (Odocoileus hemionus columbianus) and four fawns were inoculated with bluetongue virus (BTV) serotype 10 or 17, or epizootic hemorrhagic disease virus (EHDV) serotype 1. Animals were bled at irregular intervals thereafter and the presence of virus-specific antibodies in serum determined by agar gel immunodiffusion (AGID), serum neutralization (SN) and competitive enzyme-linked immunosorbent assay (C-ELISA) tests. Serum antibodies to BTV were detected in all three tests for 692 days after inoculation (DAI) of adult deer, but both the SN and AGID tests gave either erroneous or misleading results. Serum from one deer was negative by the AGID test at 409 DAI with BTV-10 but was positive at 248 and 692 DAI; also one adult and one fawn had antibodies by the SN test to serotypes of BTV with which they were not inoculated. The AGID test for EHDV had false positive results with some sera from animals inoculated only with BTV, and it consistently had false negative results with serum samples collected from an EHDV-inoculated deer at 140 DAI and thereafter. The C-ELISA was the most useful test for the detection of antibodies to BTV because it rapidly gave quantitative and accurate results.
三只成年黑尾鹿（哥伦比亚白尾鹿，Odocoileus hemionus columbianus）和四只幼鹿接种了蓝舌病毒（BTV）血清型10或17，或 epizootic hemorrhagic disease virus（EHDV）血清型1。此后，动物不定期采血，并通过琼脂凝胶免疫扩散（AGID）、血清中和（SN）和竞争酶联免疫吸附测定（C-ELISA）试验检测血清中病毒特异性抗体。在成年鹿接种病毒后692天（DAI）的所有三项试验中均检测到针对BTV的血清抗体，但SN和AGID试验均给出了错误或误导性结果。一只鹿的血清在接种BTV-10后409 DAI时AGID试验呈阴性，但在248和692 DAI时呈阳性；此外，一只成年鹿和一只幼鹿通过SN试验对未接种的BTV血清型产生了抗体。EHDV的AGID试验对仅接种BTV的动物的一些血清产生了假阳性结果，并且在140 DAI及之后从接种EHDV的鹿采集的血清样本中一直产生假阴性结果。C-ELISA是检测BTV抗体最有用的试验，因为它能快速给出定量和准确的结果。
